FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002258576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52449313
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG3162800.6030202912286TruSeq Adapter, Index 18 (97% over 39bp)
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG1303840.2485904820145118No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG1259060.240052715275794No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT1244880.23734915269528886TruSeq Adapter, Index 18 (97% over 40bp)
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC1094620.20870054103473193No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC769310.14667684970439937No Hit
CCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCC747880.1425910001909844No Hit
CGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGACG734870.14011051012241096No Hit
CCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGAC693290.13218285623683956No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC654280.12474519923645139No Hit
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC650990.12411792695931022No Hit
CTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCACGTG625170.11919507887548499No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA578760.1103465359021957No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG555590.10592893752488235No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA541780.1032959192430223No Hit
GCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAG540250.10300420903511166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)