FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19214777
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA9207454.7918588906860595RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGC7353013.8267475079205973RNA PCR Primer, Index 31 (100% over 50bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC5752362.993716762885148RNA PCR Primer, Index 31 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC5697082.964947238263551RNA PCR Primer, Index 30 (100% over 36bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3942322.0517125959879734RNA PCR Primer, Index 1 (100% over 33bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC2203021.1465238446431099RNA PCR Primer, Index 30 (100% over 36bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1708790.8893103469272634RNA PCR Primer, Index 2 (100% over 34bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC1642160.8546339101411378RNA PCR Primer, Index 31 (100% over 44bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1616650.8413576696726691No Hit
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG1591980.8285185927476546Illumina Small RNA Adapter 2 (100% over 21bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1591600.8283208282875206RNA PCR Primer, Index 2 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1515750.7888460011791967RNA PCR Primer, Index 7 (100% over 35bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA1450820.7550543001357758RNA PCR Primer, Index 31 (100% over 40bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1278000.6651131053979966RNA PCR Primer, Index 7 (100% over 35bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1193440.6211053086902856RNA PCR Primer, Index 7 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1129240.5876935235834378No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1106810.5760202161076342No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC964850.5021395772638944RNA PCR Primer, Index 2 (100% over 34bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA910360.4737811945462599RNA PCR Primer, Index 1 (100% over 32bp)
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG902250.4695604846207687Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT715470.37235404813701456No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC700480.36455276061751846RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA659270.34310572534877715No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC655680.34123737163330076RNA PCR Primer, Index 2 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA629610.32766968880252945No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT608140.31649599680495905RNA PCR Primer, Index 31 (100% over 39bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC602620.3136232078051179RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG595190.3097563921767086Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA589640.3068679901931727No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA586540.3052546485447112RNA PCR Primer, Index 1 (100% over 32bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC550370.28643059453669434RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG544420.2833340194372279No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG541420.28177272106774903No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC536100.2790040186258732No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG513610.26729948518268Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA475180.24729925306965572RNA PCR Primer, Index 31 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG472930.24612827929254658No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA465660.24234473291050943RNA PCR Primer, Index 1 (100% over 32bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA449580.23397617365010273RNA PCR Primer, Index 31 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG448080.2331955244653633No Hit
CCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTAT442940.23052049992565615RNA PCR Primer, Index 31 (100% over 48bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC428070.22278166434093927RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC392130.20407730987458245RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA380890.198227645316935RNA PCR Primer, Index 1 (100% over 28bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC377470.19644776517572907RNA PCR Primer, Index 30 (100% over 36bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC374590.19494891874102938RNA PCR Primer, Index 1 (100% over 26bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA371490.19333557709256788RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC366920.19095719924306173No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC366510.19074382179923294No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG366330.19065014389706422No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT360830.18778776355301965No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT355610.1850711043901264No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA348420.1813291926312754RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC347790.18100131997368485RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC346780.1804756828559603RNA PCR Primer, Index 1 (100% over 31bp)
TTCCGGGTTCGAGTCCCGGCGGAGTCGCCTGGAATTCTCGGGTGCCAAGG335340.17452193174034752Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC329210.17133167873871238RNA PCR Primer, Index 1 (100% over 26bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCTGGAATTCTCGGGTGCCAAGGA329210.17133167873871238RNA PCR Primer, Index 1 (100% over 22bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA326800.17007743571523104RNA PCR Primer, Index 1 (100% over 28bp)
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTA320450.16677268749983412RNA PCR Primer, Index 31 (100% over 47bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT311920.16233339580261585RNA PCR Primer, Index 1 (100% over 25bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATG289570.15070172294999834RNA PCR Primer, Index 31 (100% over 49bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA289350.15058722773623653No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA285670.1486720350696758RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC285250.14845345329794876RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA285010.14832854942839047RNA PCR Primer, Index 1 (100% over 32bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTAT284740.14818803257513735RNA PCR Primer, Index 31 (100% over 48bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT281770.1466423471893533RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC277840.14459704632533596No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA274920.1430773825790432RNA PCR Primer, Index 1 (100% over 23bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG270880.140974834108145RNA PCR Primer, Index 31 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC265040.13793550661555948No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA262400.13656156405041806RNA PCR Primer, Index 1 (100% over 28bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG255300.1328664912426514RNA PCR Primer, Index 31 (100% over 37bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG253310.13183082999089712RNA PCR Primer, Index 31 (100% over 37bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT252130.1312167192989021RNA PCR Primer, Index 1 (100% over 30bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA251790.13103977215036114RNA PCR Primer, Index 1 (100% over 23bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA247320.12871343757983764RNA PCR Primer, Index 7 (100% over 35bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG246740.1284115865617384RNA PCR Primer, Index 1 (100% over 29bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG239570.12468008345868391RNA PCR Primer, Index 31 (100% over 37bp)
GTTCGATTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC238600.12417526365255242RNA PCR Primer, Index 1 (100% over 27bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC233720.12163555163820013RNA PCR Primer, Index 1 (100% over 24bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC232800.12115675347155992RNA PCR Primer, Index 30 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT231610.12053743845166665No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC230870.12015231818719521RNA PCR Primer, Index 1 (100% over 27bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG226710.11798731778151783RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA224810.11699849548084788RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA224270.1167174617743417RNA PCR Primer, Index 1 (100% over 22bp)
TCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC208660.10859350592515332RNA PCR Primer, Index 2 (100% over 34bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCG205580.10699057293248836RNA PCR Primer, Index 31 (100% over 45bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCG202190.10522630577497726RNA PCR Primer, Index 31 (100% over 45bp)
TCGTACGACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAA200760.10448208688552565RNA PCR Primer, Index 1 (100% over 23bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC199440.10379511560295496RNA PCR Primer, Index 2 (100% over 34bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA196400.10221299992188304RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA196100.10205687008493515RNA PCR Primer, Index 1 (100% over 32bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC194180.10105763912846867RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)