FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14421767
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA9122106.325230465864551RNA PCR Primer, Index 2 (97% over 36bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4720033.2728513780592903RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT3969932.752734807045489RNA PCR Primer, Index 3 (100% over 34bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2808031.947077636187022RNA PCR Primer, Index 3 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG2380511.65063684637257RNA PCR Primer, Index 2 (97% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1964511.3621839820321602RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1680641.165349571935256No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC1530191.061028097319836RNA PCR Primer, Index 4 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1327720.9206361467356948No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1272180.8821249157610159No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG1203350.8343984478462314No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT1127480.781790469919532No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA1127230.7816171208424044RNA PCR Primer, Index 2 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG941270.6526731433117731No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT916190.6352827638943273No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG902280.6256376212429448Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA875880.6073319586982648No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA873310.6055499301853927RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC855250.5930271928536912No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG828230.5742916245977348No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG779770.5406896394873111No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC738040.5117542115331638No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT729430.5057840693168875RNA PCR Primer, Index 3 (100% over 34bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG716840.49705420979273895RNA PCR Primer, Index 2 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC685310.4751914241853997No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGCC683270.4737768957160381RNA PCR Primer, Index 4 (100% over 50bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG659710.45744047868752835RNA PCR Primer, Index 2 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC648360.44957043058593305RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT629420.4364375045027423No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC613590.42546104093901943RNA PCR Primer, Index 1 (100% over 27bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC602140.4175216532065731RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG595730.41307698286902017No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA506150.35096254155264056RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA504730.3499779187945555No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA492630.3415878234615772RNA PCR Primer, Index 1 (100% over 32bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC490630.340201030844556RNA PCR Primer, Index 4 (100% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC465180.3225540947929612No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC450240.31219475394381285RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT450170.3121462162022171No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA441970.3064603664724302RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA437640.3034579604565793RNA PCR Primer, Index 1 (100% over 32bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT427650.2965309313345584RNA PCR Primer, Index 3 (100% over 34bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC408470.2832315901373251RNA PCR Primer, Index 1 (100% over 24bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC406900.2821429579329634No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT396130.27467507969030425RNA PCR Primer, Index 1 (100% over 25bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC394860.2737944663784958RNA PCR Primer, Index 1 (100% over 26bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA387480.26867720162168757RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC377990.26209687065392195RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC352640.2445192742331782No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG336060.23302276343807243RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG329090.22818979116775356No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC328790.2279817722752004No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA304950.2114512042803077RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC297550.20632007159732924RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATTGGAATT291140.2018754012597763No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAT288960.20036379730722317No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA268190.185961955979458RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTGGAAT266210.18458903128860701No Hit
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG265000.1837500217553092No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA249540.1730301148257353No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC245580.1702842654440333No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA242330.16803072744137387RNA PCR Primer, Index 1 (100% over 32bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT240360.16666473671360799RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC236890.1642586515230762RNA PCR Primer, Index 1 (100% over 26bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA235250.1631214815771188RNA PCR Primer, Index 4 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG230740.15999426422573598No Hit
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC224960.15598643356254474RNA PCR Primer, Index 4 (100% over 38bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC223450.15493940513669371RNA PCR Primer, Index 1 (100% over 31bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC218410.15144468774180028RNA PCR Primer, Index 4 (100% over 38bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG212320.14722190422297074RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT207660.14399067742531133RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC203590.1411685544496732RNA PCR Primer, Index 1 (100% over 31bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA197720.13709831811871595RNA PCR Primer, Index 1 (100% over 22bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG197670.13706364830329043No Hit
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA194710.13501119523009905RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA190580.13214746847595027RNA PCR Primer, Index 1 (100% over 32bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC190060.13178690239552476RNA PCR Primer, Index 1 (100% over 33bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC189600.13146794009360988RNA PCR Primer, Index 1 (100% over 26bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG188370.13061506263414185RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA181110.1255810054343549RNA PCR Primer, Index 1 (100% over 23bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA179880.12472812797488685RNA PCR Primer, Index 2 (97% over 36bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC179420.12440916567297197RNA PCR Primer, Index 1 (100% over 24bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC177830.12330666554244013RNA PCR Primer, Index 1 (100% over 31bp)
TCGTACGACTCTTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA175010.12135128795244023RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA173190.12008930667095094RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA172700.11974954247978074RNA PCR Primer, Index 2 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTTGGAA166840.11568624011190862No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC163590.11343270210924916RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA162290.11253128690818538RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTGGAA160930.11158826792861097No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA159930.11089487162010037RNA PCR Primer, Index 4 (100% over 40bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA159480.11058284328127059RNA PCR Primer, Index 2 (97% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATGGAAT158230.10971609789563233No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT157000.10886322043616431No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTTGGA156540.10854425813424944No Hit
GTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT155880.10808661657063243RNA PCR Primer, Index 3 (100% over 34bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT155260.10765671085935587RNA PCR Primer, Index 1 (100% over 25bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA151610.10512581433329217RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA149570.10371128586393055No Hit
GTCTACGGCCATACCACCCTGAACGCTGGAATTCTCGGGTGCCAAGGAAC148560.10301095559233484RNA PCR Primer, Index 1 (100% over 24bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAAT147540.10230369135765403RNA PCR Primer, Index 4 (100% over 41bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC146350.1014785497505264No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA145960.10120812519020728RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGAC144870.10045232321393072RNA PCR Primer, Index 4 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)