FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10316005
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA6046695.861464782151618RNA PCR Primer, Index 2 (100% over 36bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2193872.126666282150891RNA PCR Primer, Index 1 (100% over 33bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2138942.0734189252525566RNA PCR Primer, Index 2 (100% over 34bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2001201.9398982454932892RNA PCR Primer, Index 1 (100% over 32bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1472391.4272870166309535RNA PCR Primer, Index 2 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG1388981.3464320732686732RNA PCR Primer, Index 2 (100% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC1359501.317855119302482RNA PCR Primer, Index 2 (100% over 50bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA1052931.020676124139141RNA PCR Primer, Index 2 (100% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC903460.8757847635785364No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA898970.8714323034934551No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA716220.6942803924581269No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT614710.5958798973052069No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC579570.561816323276307No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC573480.5559128751876332RNA PCR Primer, Index 2 (100% over 50bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG558550.5414402183791108RNA PCR Primer, Index 2 (100% over 35bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG547270.5305057529537839RNA PCR Primer, Index 2 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC531200.514928017192702No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA494660.47950732866065887RNA PCR Primer, Index 1 (100% over 23bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC459810.4457248712074102RNA PCR Primer, Index 1 (100% over 33bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC425130.41210720622954333RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT414420.4017252802804962No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA404150.391769876032437No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA393570.38151396785868175No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG388590.3766865176975001No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC355890.34498820037407896RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG353320.3424969258933085No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC352930.34211887256743284RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA344930.3343639325494705No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC334530.3242825105261194RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC323210.3133092704007026No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC322170.3123011281983675No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA317130.3074155159870512RNA PCR Primer, Index 1 (100% over 32bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA306410.2970238963629816RNA PCR Primer, Index 1 (100% over 22bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA292470.28351091338168216RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG291470.2825415458794368No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC285470.27672534086596506No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC274290.26588781219086266No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC269190.2609440379294116RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA260490.25251054065987755No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG255910.24807083749959408No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT255260.24744074862313464RNA PCR Primer, Index 1 (100% over 25bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC249610.24196382223544868RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC240660.2332879830903533RNA PCR Primer, Index 1 (100% over 31bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA234080.22690954492557924No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC229570.22253769749045293No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG228960.22194638331408334No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA225780.21886379465694325RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG224700.21781687775451836No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG224610.21772963467931628Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA217050.21040121636234183RNA PCR Primer, Index 1 (100% over 28bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA210210.20377074264698397RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC207800.2014345669665728RNA PCR Primer, Index 1 (100% over 27bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG204020.1977703578080856RNA PCR Primer, Index 2 (100% over 38bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT184780.17911972706488608RNA PCR Primer, Index 2 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA181000.17545551790639882No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT179940.17442798835401882RNA PCR Primer, Index 2 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT176600.17119030089651954No Hit
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA176160.17076377919553162RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA174060.16872810744081648No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC170450.16522869075771096No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT166580.16147723852402165No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA162600.15761915586508538RNA PCR Primer, Index 1 (100% over 23bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC159460.15457534190803512RNA PCR Primer, Index 1 (100% over 33bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG157660.1528304804039936RNA PCR Primer, Index 2 (100% over 38bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC156970.15216161682744433No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA153390.14869128116940616RNA PCR Primer, Index 1 (100% over 28bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG149990.14539543166177218No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA141580.13724305096788922No Hit
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA135760.13160133210482158RNA PCR Primer, Index 2 (100% over 36bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA134560.13043809110212723RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA128530.12459280506358808RNA PCR Primer, Index 1 (100% over 32bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA128330.12439893156313903RNA PCR Primer, Index 2 (100% over 40bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG127490.12358466286125297No Hit
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC124870.12104492000537029RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA122830.11906741030078989RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA122530.1187766000501163RNA PCR Primer, Index 1 (100% over 23bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC122180.11843732142433044RNA PCR Primer, Index 2 (100% over 44bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC120370.11668276624526647RNA PCR Primer, Index 1 (100% over 24bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA120160.11647919906979495No Hit
CGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG120040.11636287496952552RNA PCR Primer, Index 1 (100% over 29bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG115510.1119716401843543No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC114840.11132216395784997No Hit
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT114350.11084717388174976RNA PCR Primer, Index 2 (100% over 37bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG113170.10970332022910033No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG112600.10915078075282049No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT111810.1083849804260467RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT111570.10815233222550784RNA PCR Primer, Index 1 (100% over 30bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT110880.10748346864895858RNA PCR Primer, Index 1 (100% over 25bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT110250.10687276712254405RNA PCR Primer, Index 1 (100% over 30bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTTGGAATTCTCGGGTGC107610.10431363691661646No Hit
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG106920.1036447733400672RNA PCR Primer, Index 2 (100% over 35bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA105780.10253969438750755RNA PCR Primer, Index 2 (100% over 36bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC105360.10213256003656454RNA PCR Primer, Index 1 (100% over 27bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA104420.10122135458445396RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC104400.10120196723440907No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA103870.10068820245821904RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)