FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16810901
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC5842463.475399682622603RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT4938912.937921054915498RNA PCR Primer, Index 12 (100% over 36bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4328162.574615126220778RNA PCR Primer, Index 1 (100% over 32bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT3475102.067170581755255RNA PCR Primer, Index 12 (100% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2241111.3331290214605391RNA PCR Primer, Index 2 (100% over 34bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT2166461.2887233111419787RNA PCR Primer, Index 1 (97% over 35bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT2011471.1965271819755527RNA PCR Primer, Index 1 (97% over 35bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1702141.0125215775168743RNA PCR Primer, Index 2 (100% over 34bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT1380180.8210029908569446RNA PCR Primer, Index 1 (97% over 35bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1210430.7200268444862057RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC1123020.668030821191559RNA PCR Primer, Index 12 (100% over 50bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1031740.6137327202152937RNA PCR Primer, Index 2 (100% over 34bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCC1024750.6095747039376413RNA PCR Primer, Index 12 (100% over 50bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT996400.5927106465025284RNA PCR Primer, Index 1 (100% over 30bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC952970.5668762191865861RNA PCR Primer, Index 12 (100% over 44bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA932220.5545330378187343RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC873090.5193594323112128RNA PCR Primer, Index 1 (100% over 24bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC742510.4416836432502934RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA729060.43368288231546903RNA PCR Primer, Index 1 (100% over 28bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA655310.38981253889961043RNA PCR Primer, Index 12 (100% over 39bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT632270.37610714619043917RNA PCR Primer, Index 12 (100% over 38bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA612990.36463839743033405RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA600360.3571254152290826RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA594080.3533897439524509RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA550830.32766239001704905RNA PCR Primer, Index 1 (100% over 32bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG537110.3195010190114141RNA PCR Primer, Index 12 (100% over 37bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC516730.3073779329257843RNA PCR Primer, Index 1 (100% over 33bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC514600.3061108979227229RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC510370.3035946734800235RNA PCR Primer, Index 1 (100% over 26bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA509250.3029284391122165RNA PCR Primer, Index 12 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA501130.29809823994561624No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT495610.29481465627571063RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA454760.2705149474141808RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC451770.268736339592982RNA PCR Primer, Index 1 (100% over 33bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA423870.2521399656092199RNA PCR Primer, Index 1 (100% over 32bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT412430.2453348574237633RNA PCR Primer, Index 12 (100% over 38bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG408280.24286622115019296RNA PCR Primer, Index 12 (100% over 37bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC406070.2415515979780025RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT405980.2414980612877323RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA404280.24048681269373962RNA PCR Primer, Index 1 (100% over 32bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT402040.23915434395812576RNA PCR Primer, Index 12 (100% over 36bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC393380.23400292464990424RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG379810.2259307814613863RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA372470.2215645669437944RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA371080.22073772250517684No Hit
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT368170.21900670285310703RNA PCR Primer, Index 12 (100% over 36bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA358630.2133318136844658RNA PCR Primer, Index 1 (100% over 32bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA357620.21273101304921135RNA PCR Primer, Index 1 (100% over 28bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA354330.2107739495937785RNA PCR Primer, Index 1 (100% over 23bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA350100.20825772515107904RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA339790.20212479985457057No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC339210.2017797856283848No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT336580.20021532456826674RNA PCR Primer, Index 1 (100% over 25bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA333270.19824636407055157RNA PCR Primer, Index 12 (100% over 40bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG332880.1980143717460474RNA PCR Primer, Index 12 (100% over 45bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA332310.1976753060410028RNA PCR Primer, Index 1 (100% over 23bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA325400.19356487793247965RNA PCR Primer, Index 12 (100% over 40bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC319810.19023965461458608RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC316370.1881933633420362No Hit
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT313910.18673002714131742RNA PCR Primer, Index 12 (100% over 36bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG312880.18611732946378068RNA PCR Primer, Index 1 (100% over 29bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG309040.18383309734558545RNA PCR Primer, Index 12 (100% over 37bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG306720.1824530404408425No Hit
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT306040.18204854100324547RNA PCR Primer, Index 1 (97% over 35bp)
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG305160.18152507114282573RNA PCR Primer, Index 1 (100% over 29bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG290980.1730900681646986RNA PCR Primer, Index 12 (100% over 37bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC277210.16489895455335796RNA PCR Primer, Index 1 (100% over 26bp)
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGAA275360.1637984781422483RNA PCR Primer, Index 1 (100% over 23bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT272730.16223401708213023RNA PCR Primer, Index 12 (100% over 36bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA269830.16050894595120155RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG269220.1601460861615924No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA265270.15779642031084473No Hit
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC263390.15667809833631166RNA PCR Primer, Index 12 (100% over 44bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG257950.1534421028355351Illumina Small RNA Adapter 2 (100% over 21bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA256870.15279966255229271RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC254670.15149098790124335RNA PCR Primer, Index 1 (100% over 27bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCG254400.15133037783043277No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA253450.15076526832202508No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC242430.14420999802449613RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA238690.14198525111771224RNA PCR Primer, Index 1 (100% over 32bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA238310.14175920731434918RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT229890.1367505525135149RNA PCR Primer, Index 1 (97% over 35bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA227480.13531695891850176RNA PCR Primer, Index 1 (100% over 22bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA225160.13393690201375882RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC218350.129885959116647RNA PCR Primer, Index 1 (100% over 24bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT217860.12959448158073145RNA PCR Primer, Index 1 (97% over 35bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA214610.12766121220986312RNA PCR Primer, Index 1 (100% over 28bp)
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA212360.1263227949531081No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC210010.12492489248494176RNA PCR Primer, Index 1 (100% over 31bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG207680.12353888705905769Illumina Small RNA Adapter 2 (100% over 21bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG206350.12274773374728695No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG201440.11982700986699046No Hit
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG200100.1190299080340786RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC196340.11679326408501245RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC194530.11571658175846733RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG193750.11525259710945893RNA PCR Primer, Index 12 (100% over 45bp)
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT191050.11364649640135292RNA PCR Primer, Index 12 (100% over 36bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC189090.11248058625769077RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC188950.11239730696171489RNA PCR Primer, Index 1 (100% over 27bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT188820.11231997618688017RNA PCR Primer, Index 1 (100% over 30bp)
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT188810.11231402766573903RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGATTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG188580.11217721167949296RNA PCR Primer, Index 1 (100% over 29bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT187260.11139200688886336RNA PCR Primer, Index 1 (100% over 25bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA185600.11040455237943522RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA185320.1102379937874835No Hit
CCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC184870.1099703103361325RNA PCR Primer, Index 1 (100% over 31bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA183490.10914941441865608RNA PCR Primer, Index 1 (100% over 23bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA183030.10887578244616394RNA PCR Primer, Index 1 (100% over 28bp)
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAA182240.1084058492760144No Hit
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC178980.10646663138400493RNA PCR Primer, Index 1 (100% over 33bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT177970.10586583074875047RNA PCR Primer, Index 1 (100% over 30bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT177230.1054256401843066RNA PCR Primer, Index 12 (100% over 38bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC175010.10410506849097499RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC174540.10382548799734172No Hit
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG174490.10379574539163607No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC173950.10347452525001485RNA PCR Primer, Index 2 (100% over 34bp)
CGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA173900.10344478264430919RNA PCR Primer, Index 12 (100% over 40bp)
GTTAAAGACTTTTTCTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCA170000.10112485939926719RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC169850.10103563158215018RNA PCR Primer, Index 12 (100% over 44bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT169830.1010237345398679RNA PCR Primer, Index 12 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph