FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11314567
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG3617673.1973561162349386RNA PCR Primer, Index 10 (97% over 36bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2224851.9663589424146766RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGC2202861.9469238195328198RNA PCR Primer, Index 47 (100% over 50bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2094071.850773432160506RNA PCR Primer, Index 1 (100% over 33bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1817351.60620375485867RNA PCR Primer, Index 3 (100% over 34bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG1554781.374140079775037RNA PCR Primer, Index 10 (97% over 36bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTATGCC1117030.987249445780824RNA PCR Primer, Index 47 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1087440.9610973181739964No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC944590.8348441438368785RNA PCR Primer, Index 5 (97% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT941340.8319717405005425RNA PCR Primer, Index 3 (100% over 34bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC735330.6498967216332715RNA PCR Primer, Index 5 (97% over 35bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA734630.6492780501454453RNA PCR Primer, Index 47 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC732950.6477932385746622No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC727260.6427643231950458RNA PCR Primer, Index 5 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC699990.6186626496621568No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT648360.5730312083529135No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA639550.5652447857704144No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTC634230.5605428824629347RNA PCR Primer, Index 47 (100% over 44bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC574280.5075580886126707RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC570060.5038283833574895No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC446030.3942086338787865RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT432610.38234781764074577No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA426920.3773189022611294No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA411470.3636639387083924RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC410630.36292153292300094No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT404190.3572297552349993RNA PCR Primer, Index 3 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA400680.35412755963175613No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG377130.3333136831484581No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG364470.3221245673829144No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT332500.2938689567174776RNA PCR Primer, Index 3 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG328860.290651864980781No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG312620.27629868646321154No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC311530.2753353265750249RNA PCR Primer, Index 1 (100% over 24bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC309040.2731346237111858RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG305310.2698379884974829No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC302470.26732794988973063No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC294050.2598862157075918RNA PCR Primer, Index 1 (100% over 26bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC291820.2579153051106596RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG288300.25480427134330463Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC287980.2545214500917269No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA269070.23780848175630584RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAA256700.22687567275000448RNA PCR Primer, Index 47 (100% over 38bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG250390.22129879119545626RNA PCR Primer, Index 47 (100% over 45bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC248320.21946929122431286RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC242960.214732035260386No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT226890.20052910553271722RNA PCR Primer, Index 1 (100% over 25bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA218230.19287525541189512RNA PCR Primer, Index 1 (100% over 32bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC217320.19207098247772098RNA PCR Primer, Index 1 (100% over 24bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA217030.19181467571847866RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT216590.1914257964975593No Hit
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTC209040.1847529825931474RNA PCR Primer, Index 47 (100% over 44bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC202880.17930867350027624RNA PCR Primer, Index 1 (100% over 31bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG200160.1769046928618656RNA PCR Primer, Index 10 (97% over 36bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT199310.17615344891236226No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA198990.17587062766078454RNA PCR Primer, Index 1 (100% over 28bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG197360.17443000691056051RNA PCR Primer, Index 1 (100% over 29bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC195920.17315731127846076RNA PCR Primer, Index 1 (100% over 26bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAG194080.17153108908188885RNA PCR Primer, Index 47 (100% over 39bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTAT193510.17102731372751603RNA PCR Primer, Index 47 (100% over 48bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT192630.1702495552856773RNA PCR Primer, Index 1 (100% over 30bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA190950.16876474371489425RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCG186800.1650969056084957RNA PCR Primer, Index 47 (100% over 45bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA184500.1630641278627808RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA182150.1609871592965069RNA PCR Primer, Index 1 (100% over 32bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA178120.15742537915944993RNA PCR Primer, Index 47 (100% over 37bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA176430.15593172942455508No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA164150.14507846389525997RNA PCR Primer, Index 1 (100% over 23bp)
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA162380.1435141088474707No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA161280.14254191079517226RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT161260.14252423446694867No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAG161030.14232095669237718RNA PCR Primer, Index 47 (100% over 39bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA160500.14185253399445158RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC160380.14174647602510992No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG159140.14065054367524624No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA157940.1395899639818298No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA157900.13955461132538258RNA PCR Primer, Index 1 (100% over 32bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAA156870.1386442804218668RNA PCR Primer, Index 47 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA154100.13619610896289713No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA153070.13528577805938133RNA PCR Primer, Index 1 (100% over 28bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTC152720.13497644231546818RNA PCR Primer, Index 47 (100% over 44bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG152620.13488806067435016No Hit
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC151130.13357117422169137RNA PCR Primer, Index 5 (97% over 35bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC147610.13046014045433643No Hit
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG147090.13000055592052262RNA PCR Primer, Index 10 (97% over 36bp)
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGATCTCGTA144600.12779985305668348RNA PCR Primer, Index 47 (100% over 47bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG144220.12746400282043494No Hit
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA144210.12745516465632312RNA PCR Primer, Index 47 (100% over 37bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAA139710.12347799080601139RNA PCR Primer, Index 47 (100% over 38bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG139640.12341612365722877RNA PCR Primer, Index 10 (97% over 36bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA138160.1221080753686818RNA PCR Primer, Index 1 (100% over 23bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT137120.12118890630105422RNA PCR Primer, Index 1 (100% over 30bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG128080.11319920594398353Illumina Small RNA Adapter 2 (100% over 21bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC126120.11146692577806998No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG125180.11063613835156041No Hit
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG124400.10994676155083973No Hit
CGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA124390.10993792338672792RNA PCR Primer, Index 47 (100% over 40bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA121290.10719809251206873RNA PCR Primer, Index 1 (100% over 22bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA120610.10659709735246607RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA119620.10572211910539749RNA PCR Primer, Index 1 (100% over 22bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG119130.1052890490639191RNA PCR Primer, Index 1 (100% over 29bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGAAGA118120.10439639448862692RNA PCR Primer, Index 47 (100% over 40bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC116610.10306183170774454No Hit
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC115560.10213382447600514RNA PCR Primer, Index 5 (97% over 35bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGA113540.10034851532542076RNA PCR Primer, Index 47 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)