FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15463445
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA300726619.447581053251717RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGC4715533.049469248281997RNA PCR Primer, Index 28 (100% over 50bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2529001.6354699745108545RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2133261.3795502877916272RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1942851.2564147251792857No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA1833141.185466757245879RNA PCR Primer, Index 2 (97% over 36bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1044990.6757808496101613RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC949320.61391235911532No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC788040.5096147721287203No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA747920.48366971266752007No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC714150.4618311120193463RNA PCR Primer, Index 2 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA639640.41364650632507827RNA PCR Primer, Index 7 (100% over 35bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC628100.4061837449546333RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG583400.37727686165663604Illumina Small RNA Adapter 2 (100% over 21bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG573190.3706741932344313Illumina Small RNA Adapter 2 (100% over 21bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGA535280.3461583107774497RNA PCR Primer, Index 28 (100% over 40bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG508490.32883358139146873No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC493120.3188940110046629RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG478300.3093101181528437No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA408910.2644365469660868RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA400130.25875864013484706RNA PCR Primer, Index 1 (100% over 32bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG383060.2477197028217192Illumina Small RNA Adapter 2 (100% over 21bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA373810.2417378533696728No Hit
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG369210.23876309580433078Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA343130.22189751378169612RNA PCR Primer, Index 1 (100% over 32bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA342050.22119909244026797RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA330260.213574659463011RNA PCR Primer, Index 1 (100% over 32bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA329630.21316724701384457RNA PCR Primer, Index 7 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC325570.21054170011921666RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC313130.2024969209642483No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC302650.19571964720668647RNA PCR Primer, Index 1 (100% over 31bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC299780.19386365716048398RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC298440.1929970973479713RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC295120.19085009840950706RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC293870.19004174037544674No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT285420.18457724006519893RNA PCR Primer, Index 1 (100% over 25bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG283450.18330326780351985No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG282170.18247550917664207No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC282020.18237850621255483RNA PCR Primer, Index 1 (100% over 33bp)
TCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC274540.17754129173673783RNA PCR Primer, Index 2 (100% over 34bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA274530.17753482487246536RNA PCR Primer, Index 2 (97% over 36bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC271680.1756917685548078RNA PCR Primer, Index 1 (100% over 26bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAA260810.1686622870906192RNA PCR Primer, Index 28 (100% over 38bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC256390.1658039330821819RNA PCR Primer, Index 1 (100% over 33bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAG239360.15479086322614397RNA PCR Primer, Index 28 (100% over 39bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA238900.15449338746960978RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA234220.15146689499008792No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA234090.15138282575454565RNA PCR Primer, Index 1 (100% over 28bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGCC231040.14941043215143843RNA PCR Primer, Index 28 (100% over 50bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA224810.14538157570968177RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA217080.14038268962705272RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC211730.1369229172412745No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC210470.1361080923429417RNA PCR Primer, Index 1 (100% over 27bp)
GGGGATGTAGCTCAGTGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC208160.1346142466959982RNA PCR Primer, Index 1 (100% over 31bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC203530.13162008853783877RNA PCR Primer, Index 1 (100% over 24bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA201340.13020384526216505RNA PCR Primer, Index 7 (100% over 35bp)
GTAGTCGTGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA195640.12651773262684998RNA PCR Primer, Index 2 (97% over 36bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA187040.12095622935251492RNA PCR Primer, Index 1 (100% over 32bp)
AGTTGGTCCGAGTGTTGTGGGTTTGGAATTCTCGGGTGCCAAGGAACTCC186380.12052941631053107RNA PCR Primer, Index 1 (100% over 27bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC183750.11882863100686815RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG182710.11815607712252994No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT180090.11646175868313949No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA178740.11558873200635433RNA PCR Primer, Index 7 (100% over 35bp)
TCGTACGACTCTTAGCGGTGGATCACTTGGAATTCTCGGGTGCCAAGGAA172200.1113594027721507RNA PCR Primer, Index 1 (100% over 23bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA169790.1098008884824824RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG168120.10872092214897779No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG165850.10725294395912426No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACACCAGTCA164960.10667739303887329RNA PCR Primer, Index 1 (96% over 32bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT164450.10634758296097668RNA PCR Primer, Index 1 (100% over 30bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA162520.10509947815638752RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA161380.10436225562932451No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC158860.10273260583265889RNA PCR Primer, Index 1 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)