FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002258624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16531092
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2821241.7066265192886227RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA2782161.6829862177283872RNA PCR Primer, Index 3 (100% over 36bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2684001.6236072002986859RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2422301.4652994490624092RNA PCR Primer, Index 3 (100% over 34bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1565710.9471304134052366RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1445910.874660911692948No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1417290.8573480808164398RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1166050.7053678002638907No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1142020.6908315554713506No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA1140350.6898213378765299RNA PCR Primer, Index 3 (100% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1081060.6539555886568171RNA PCR Primer, Index 3 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC1061520.6421354378766992RNA PCR Primer, Index 3 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1012550.6125124704405492No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG991710.5999059227303314No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA982580.5943829965981678No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG922520.5580514584275498No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC921940.5577006044125821No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG885780.535826671341494Illumina Small RNA Adapter 2 (100% over 21bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT832960.5038747591508171RNA PCR Primer, Index 1 (97% over 35bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA809790.489858746173574No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC789020.4772945429134385No Hit
ACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAGTC784160.47435462823629554RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG660250.39939890238346015No Hit
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC647320.39157727753254296RNA PCR Primer, Index 1 (100% over 33bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT626310.37886789330069665RNA PCR Primer, Index 1 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC536750.324691194023964RNA PCR Primer, Index 1 (100% over 26bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC529790.32048094584435194RNA PCR Primer, Index 3 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG518900.3138933592529761No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT512090.3097738491806833RNA PCR Primer, Index 1 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC499080.30190383067252907RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC488420.2954553758457094RNA PCR Primer, Index 1 (100% over 33bp)
AGAGGTGTAGAATAAGTGGGAGGCCCTGGAATTCTCGGGTGCCAAGGAAC480830.29086402761535657RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC470740.2847603775963499No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG427030.25831929312352747No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT406170.24570064699900043No Hit
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCC371910.2249760632872892RNA PCR Primer, Index 1 (100% over 27bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT361540.21870303546795336RNA PCR Primer, Index 3 (100% over 34bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC358390.2167975352142496RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT336200.20337434453815878RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC334060.20207981420707113No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT331520.2005433155897989No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC327410.19805709144925213RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA324300.19617578802416683RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA322860.19530470219390225RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC320160.19367141626215617No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT298080.1803147668647661RNA PCR Primer, Index 3 (100% over 34bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTC279810.16926286539328436RNA PCR Primer, Index 3 (100% over 44bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA272370.16476225527025076RNA PCR Primer, Index 1 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA271140.16401820279023308No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT263870.15962042918882793RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT246420.14906456270402463No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC240370.1454047923754825RNA PCR Primer, Index 1 (100% over 24bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA229300.13870832005532363RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA229130.13860548353369517RNA PCR Primer, Index 1 (100% over 32bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA219980.1330704589872224RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG217820.13176383024182553RNA PCR Primer, Index 3 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA211890.12817665039913878No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC209310.1266159549532481RNA PCR Primer, Index 1 (100% over 27bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT206930.12517624365044971RNA PCR Primer, Index 3 (100% over 34bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG204660.12380307362635208RNA PCR Primer, Index 3 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC203500.12310136559641673RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA200690.1214015383859699No Hit
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC196840.11907259363144311RNA PCR Primer, Index 3 (100% over 39bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA187230.11325930555585802RNA PCR Primer, Index 1 (100% over 23bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTTGGAATTCTCGGGTGCCAAGG185130.11198897205338884Illumina Small RNA Adapter 2 (100% over 21bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG184830.11180749583875041RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA180350.10909745103348284RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATTGGAATT175960.10644184909260684No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC172400.10428833134556387RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT169920.10278812797121933RNA PCR Primer, Index 1 (100% over 30bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG169910.10278207876406471RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA166910.10096731661768019RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)