FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50423
Sequences flagged as poor quality0
Sequence length15-160
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA541910.747079705689863No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG38097.554092378478075No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA20944.152866747317693No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG18023.5737659401463615No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA14712.917319477222696No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA10192.020903159272554No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA8561.6976379826666401No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA8011.588560775836424No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATACTGG7741.5350137833924995No Hit
GAGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG6811.3505741427523155No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCATGCA6421.2732284869999801No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA6241.2375304920373638No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA5841.1582016143426612No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG5541.098704956071634No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGAGACA4780.9479800884516986No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCACACA4280.8488189913333201No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG4240.8408861035638497No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC3820.7575907819844119No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC3750.7437082283878389No Hit
GGTACAGCGTCTCNN3550.7040437895404874No Hit
GGAGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG3330.6604129068084009No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCACAGA3320.6584296848660334No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAAACAGAATTTCCTGCTG3290.6524800190389307No Hit
CCAGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA3130.6207484679610495No Hit
CAGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA3060.6068659143644765No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG3000.594966582710271No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC2590.5136544830732007No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG2140.42440949566666003No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG2100.41647660789718977No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGAATCTGG2080.4125101640124546No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATATGAG1870.3708625032227357No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG1800.35697994962616264No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCAGCA1780.35301350574142754No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTACTCCCTGATTCTGG1690.3351645082601194No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCATGCA1690.3351645082601194No Hit
GAGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATACTGG1670.33119806437538424No Hit
GGAACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGAGACA1620.32128195466354637No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCAGCAAA1600.31731551077881126No Hit
CTACAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA1570.31136584495170855No Hit
GCAGAGAGGCACAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA1560.30938262300934094No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGAGTAC1520.30144973523987073No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA1510.2994665132975031No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA1500.2974832913551355No Hit
GGAACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA1500.2974832913551355No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG1500.2974832913551355No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA1490.295500069412768No Hit
GGGGTACAGTGTCTN1480.2935168474704004No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCACTGCA1460.2895504035856653No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA1340.26575174027725446No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT1260.24988596473831387No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT1170.23203696725700573No Hit
GTTTCCCATCAGCCGCCCAAACCTGACAAACACATCACAATAAAACTACA1130.22410407948753544No Hit
AGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG1130.22410407948753544No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCAGCAGA1130.22410407948753544No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTACAGA1060.21022152589096244No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT1060.21022152589096244No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAAACAGAATTTCCAGCTG1020.20228863812149217No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCAGCTGG1010.2003054161791246No Hit
GGGGTACAGAGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG990.19633897229438946No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAAAGTGAG990.19633897229438946No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA960.19038930646728675No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTACACCTACACGCCCTGCA960.19038930646728675No Hit
CAGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCACACA930.18443964064018403No Hit
GGAGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATACTGG930.18443964064018403No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGAGAG900.17848997481308132No Hit
TGGCTACAATGTCTCCAGATTAAAAAAAACAGAATTTCCTGCTGGGGTTG900.17848997481308132No Hit
TGGATACAGTGTCTN890.17650675287071377No Hit
CAATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG880.1745235309283462No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG870.1725403089859786No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCAGAGATGAATGTGAG860.17055708704361106No Hit
CGATTCTCAGAGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG840.1665906431588759No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTACTGG810.16064097733177318No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGAGCACA810.16064097733177318No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC800.15865775538940563No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA790.15667453344703808No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGATTGG790.15667453344703808No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATACTGG770.15270808956230292No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAACGCTTCTCCCTGATTCTGG760.15072486761993537No Hit
GCAAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA760.15072486761993537No Hit
GATACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA750.14874164567756776No Hit
GAACCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC740.1467584237352002No Hit
TCTCCACTCTGACGN700.13882553596572994No Hit
CTGCAGAGAGACCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA670.13287587013862723No Hit
CAACAGTCTCCAGAATA650.12890942625389207No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGAAACTGG650.12890942625389207No Hit
CCAGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA640.12692620431152452No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCAGGAGG630.12494298236915694No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC610.12097653848442179No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTAGAGG610.12097653848442179No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTACAAA600.11899331654205422No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAAGTGCAGCCTGCAGA590.11701009459968664No Hit
GAAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA590.11701009459968664No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGAAGATCCAGCGCACA580.11502687265731908No Hit
CCTGAATGCACCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA580.11502687265731908No Hit
CTGCAGAGAGGCCTAAG580.11502687265731908No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATTCAGCGCACA580.11502687265731908No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCAACTACACGCCCTGCA570.11304365071495151No Hit
GATTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA570.11304365071495151No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACACAGCC560.11106042877258393No Hit
GGAACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG560.11106042877258393No Hit
GATACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGAGACA550.10907720683021636No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTAAGCC540.1070939848878488No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAAAACAGAATTTCCTGCT530.10511076294548122No Hit
TGGCTACAATGTCTCC530.10511076294548122No Hit
GGTACAGCGTCTCTCGGGAGA520.10312754100311365No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGAGCAGCCTGCAGA510.10114431906074609No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCAGAGGA510.10114431906074609No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCAGGAG510.10114431906074609No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)