FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46380
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA26765.769728331177232No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG23895.150927123760241No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA23775.1250539025442No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGAGACA20144.342388960758948No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC19284.156964208710652No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA10212.2013799051315224No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA9612.0720137990513154No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG7191.5502371711944805No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATACTGG6891.485554118154377No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA6821.4704614057783527No Hit
GGTACAGCGTCTCNN6471.394997843898232No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA5581.203104786545925No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGACTGC4841.0435532557136697No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC4831.0413971539456663No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC4210.9077188443294524No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCACACA4140.8926261319534282No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCAGCTGC4060.8753773178094006No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG3760.8106942647692971No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT3710.7999137559292798No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGAGAGCA3450.7438551099611902No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCACAGA3360.7244501940491591No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA3350.7222940922811557No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT3250.7007330746011212No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC3180.685640362225097No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA3160.6813281586890901No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC2380.513152220784821No Hit
GGGGTACAGTGTCTN2070.4463130659767141No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGACTGC2060.4441569642087107No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGAGTAT1980.4269081500646831No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTATGACA1910.4118154376886589No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG1880.40534713238464853No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG1870.40319103061664513No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGACTGCTGTCGGCTGC1830.3945666235446313No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC1810.3902544200086244No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC1710.3686934023285899No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACAGTGA1680.36222509702457956No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG1660.35791289348857264No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTAAGCA1640.35360068995256577No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGAGTAC1620.34928848641655885No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTAAGACA1600.344976282880552No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGAATCTGG1590.3428201811125485No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT1460.31479085812850366No Hit
TGGATACAGTGTCTN1460.31479085812850366No Hit
GGAACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGAGACA1410.3040103492884864No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGAGTAT1390.2996981457524795No Hit
GACTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC1350.2910737386804657No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT1340.28891763691246225No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA1280.2759810263044416No Hit
CAACAGTCTCCAGAATA1260.2716688227684347No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC1240.26735661923242776No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG1190.2565761103924105No Hit
CAGACCCCAGGNNNN1180.25442000862440706No Hit
GGAACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA1120.24148339801638635No Hit
CTGCAGAGAGGCCTAAG1120.24148339801638635No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCAACTGC1110.23932729624838292No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACAGTGA1110.23932729624838292No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG1080.2328589909443726No Hit
GGTACAGCGTCTCTCGGGAGA1070.2307028891763691No Hit
AGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG1070.2307028891763691No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT990.21345407503234154No Hit
TCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCC980.21129797326433808No Hit
GAGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG980.21129797326433808No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGAGCACA980.21129797326433808No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGACGGC960.2069857697283312No Hit
TGGCTACAATGTCTCC950.2048296679603277No Hit
CTGCAGAGAGACCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA940.20267356619232427No Hit
GTGTCTCCAGAGCACACGTCTGAACTCCAGTCACTCCTGAGCATCTCGTA930.20051746442432083TruSeq Adapter, Index 3 (96% over 28bp)
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACACGGCC820.17680034497628289No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTACTCCCTGATTCTGG810.17464424320827943No Hit
GGTACAGCGACTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA790.17033203967227253No Hit
GGGGTACAGAGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG780.16817593790426907No Hit
GGTACAGCGACTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGAGACA780.16817593790426907No Hit
ATCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC770.1660198361362656No Hit
GATTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA760.16386373436826218No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGACTGC740.1595515308322553No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGAGTAT730.15739542906425183No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC730.15739542906425183No Hit
TCTCCACTCTGACGN710.15308322552824494No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT710.15308322552824494No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG660.14230271668822767No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAAGCTGC650.14014661492022423No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCACACTGTGACA640.1379905131522208No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGAAAGCA640.1379905131522208No Hit
ATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC630.13583441138421734No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG630.13583441138421734No Hit
CAGATGGCTATAGTGTCTCT620.13367830961621388No Hit
CAGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA620.13367830961621388No Hit
GGGGTACAGTGTCTCTAGAG610.13152220784821042No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCAGCTGC610.13152220784821042No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGAAACTGG610.13152220784821042No Hit
GATCCATCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCC590.12721000431220353No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCCGCCGAGTAT590.12721000431220353No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGAAGATCCAGCGCACA570.12289780077619664No Hit
CCCTGATGGCAACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG570.12289780077619664No Hit
GACTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGACTGC560.12074169900819318No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCATGGAGATCCAGCGCACA560.12074169900819318No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGACTGCTGTCGACTGC540.1164294954721863No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATACTGG530.11427339370418284No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCACTCA530.11427339370418284No Hit
GATACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGAGACA510.10996119016817593No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTAGGCC510.10996119016817593No Hit
CAGACCCCAGGACCGGC510.10996119016817593No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG490.10564898663216904No Hit
GATCCATCTCCACTCTG490.10564898663216904No Hit
AGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGAGACA490.10564898663216904No Hit
GGAGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG490.10564898663216904No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTACTCTCACTGAGACA480.1034928848641656No Hit
CTACAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA480.1034928848641656No Hit
ATTTCTCATATGATGTTAAAAATATTAAACACAAACTACCACCTACCTCC470.10133678309616213No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGACTGCTGTCAGCTGC470.10133678309616213No Hit
AGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC470.10133678309616213No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCACACTGAGACA470.10133678309616213No Hit
GGGGTACAGTGTCTCAGAG470.10133678309616213No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAAATCCAGCCCTCAGA470.10133678309616213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)