FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224798
Sequences flagged as poor quality0
Sequence length35-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG2519011.205615708324807No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA114995.115259032553671No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG99364.419968149182822No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA85723.813201185063924No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA83863.730460235411347No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA69633.0974474861876No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA58462.6005569444568013No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG53932.399042696109396No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG53882.396818477032714No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC51832.3056254948887447No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCAAA25841.149476418829349No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG20040.8914670059342165No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA19740.8781216914741233No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC17930.7976049608982287No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG17440.7758076139467434No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC17150.7629071433019867No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA16910.7522308917339122No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC14700.65392040854456No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC14500.6450235322378313No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGAGGACTCGGCCCTGTATC12280.5462682052331427No Hit
CTCAGCAGAGATGCCTGATGCAACTTTAGCCACTCTGAAGATCCAGCCCT11580.5151291381595922No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA10540.46886538136460293No Hit
GGTACAGCGTCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGC10210.45418553545850054No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA8470.3767827115899608No Hit
GTTTCCCATCAGCCGCCCAAACCCACTCCACCTTACTACCAGACAACCTT7170.3189530155962242No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA6630.29493144956805667No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC6410.2851448856306551No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG6330.2815861351079636No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC6320.28114129129262716No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGACTGTGAG6150.2735789464319078No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGCTGAATGTGAG5610.24955738040374023No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC5600.24911253658840382No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT5540.2464434736963852No Hit
GATAACTTCCAATCCAGGAGGCCGCCAAGAAGAAGCCAGGCATCTTCATC5050.22464612674489987No Hit
GATAACTTCCAATCCAGGAGGCCAGCAAGCGCATCTCCAGCCACATCCCT4510.20062456071673235No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGCG4490.1997348730860595No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA4480.19929002927072306No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG4390.19528643493269515No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG4380.1948415911173587No Hit
CCTGAATGCCCCAACAGCTCTCACGGCTGCTGCTCCTCTGGACTCCCCAA4190.18638955862596643No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG4090.18194112047260205No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA3890.17304424416587336No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC3890.17304424416587336No Hit
CAGACCCCAGGACCGGCCCATGTCCCCCGGAGACTGTCCTCCGGAAACAA3880.17259940035053692No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGCATGTGAG3870.17215455653520048No Hit
CGATTCTCAGGGCGCCAGTTCTCTTCCTCCTGCGCACCTGCCCCGCTCGG3620.16103346115178963No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCCGAGAG3590.1596989297057803No Hit
GTTTCCCATCAGCCGCCCAAACCTGGAGACCTGATTGAGATTTTCCGCCT3580.15925408589044385No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGCGATGAATGTGAG3380.15035720958371515No Hit
TCTCCACTCTGAAGATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTAC3340.1485778343223694No Hit
CAGACCCCAGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAG3110.1383464265696314No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG3110.1383464265696314No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGCAGGAAAACCGCGCAGAGAG3060.13612220749294923No Hit
GGGGAACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG2970.13211861315492132No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA2970.13211861315492132No Hit
TCTCAGCTCAACAGTTCAGTGACTTGCACTCTGAACTAAACCTGAGCTCT2940.130784081708912No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCA2730.12144236158684685No Hit
CCTGAATGCCCCAACAGCTCTGACCTGGGCAGCCTTCCTGAGAAAATGCA2730.12144236158684685No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC2650.11788361106415537No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG2610.11610423580280962No Hit
GGAACGATTTTCTGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAA2540.11299032909545459No Hit
CGATTCTCAGGGCGCCGCTGCGAAGGGAGCCGCCGCCATGTCTGCGCATC2500.11121095383410885No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG2490.1107661100187724No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGCG2450.10898673475742666No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATC2380.10587282805007163No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG2380.10587282805007163No Hit
CGATTCTCAGGGCGCCACCCTTGACGCTTGCAGCTTCGGAGTCACGGGTT2370.10542798423473518No Hit
CAGACCCCAGGACCGGCCCCATCATTGAGGAGGTTGATTGAATGGCCCTT2320.10320376515805302No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGCGCA2300.10231407752738014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)