FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443699
Sequences flagged as poor quality0
Sequence length15
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN7238116.313086123700977No Hit
CTTAAGCTGTTACGC233135.2542376701322295No Hit
GAGTCTTTGTTCATC140993.1776046373780424No Hit
TAGTGGCTCATACCG140403.164307334476751No Hit
ACTGTCCCCATAGGG128432.8945298501912333No Hit
GGACTTCCTCTGCGC119862.7013808910995967No Hit
GCCATAGGTTGCCAC115182.595903979950372No Hit
GCCATTCTGGTAGTG108882.453915830326415No Hit
AACTCGTGCCTGCGG98162.2123105979504123No Hit
AGGTTCGGGGGTCAT96252.1692633970326733No Hit
GTTGCTATTCTCGGT84761.910304057480409No Hit
CTGGAGCGGAGTGTT82781.865679210455737No Hit
ATCCAGGGTGGTATG82201.852607285569722No Hit
TCACAAGCCTGATAT63701.4356579573088963No Hit
GGACTAAATAAATGT59501.340999190892925No Hit
ATCACGGCTTATAGA59341.337393142648507No Hit
GTATGCGTGTCTATA59231.3349139844804698No Hit
ATCACGGATAGTTTC58391.3159822311972758No Hit
ACGCTATTATCCATG58241.3126015609681339No Hit
CAGCGATAACTCAAC58181.311249292876477No Hit
ATCACGGCAGTAACG54991.2393537060033943No Hit
CGCTTGACCTGCTGG51721.1656550950081024No Hit
CCATGGTGTGGTGGC46501.0480077710339666No Hit
TGATGACATCAATCC44611.0054113261467796No Hit
GGCGATTGCATGGGT43310.976112184160884No Hit
ATCTACGAGGGCACG35800.8068532946885164No Hit
GTCACTAGCCGGGTA31440.7085884800281271No Hit
CAGGAATTAATTAAT30780.6937135310199031No Hit
TACCATGTTTGGTTT29710.6695980833853581No Hit
CTAGTGCGGGTTCGG29000.6535962443007535No Hit
CGACACTCGATGTGG28090.6330868449106264No Hit
CTAGAGTGTTGTTGG26740.6026608128483499No Hit
GGACGATAGTTGTAC25490.5744885609388347No Hit
TACGACTCAAAGGGT24120.5436117728460059No Hit
GGCGATTCGCTGATT23900.5386534565099312No Hit
TCTAACGAATCTCGT23260.5242292635322595No Hit
GATGTACATTAATTT23020.5188201911656326No Hit
GCGTTAGGGCCTGGA22200.5003391939129906No Hit
GGACTAATTCAGGTG21870.49290171940887856No Hit
CCTGACTGCGTCGCT18330.4131179020011314No Hit
CCATTGCAAGTTCTG18190.40996260978726573No Hit
GGAACCTTGACGGTT17270.3892278323818625No Hit
TGGATGCGAAATGAG17010.3833680039846833No Hit
TTAGCGTAGGGCGAG16630.3748036394041907No Hit
GCCATTCCGATCAGG16260.36646465283897417No Hit
TGCTACTAATGATTT15330.3455044974182948No Hit
GCATGATTAGCTTTT14620.3295026583336902No Hit
ACAGGTAAGCCATTG13840.3119231731421527No Hit
CACCTAGCGAGTTGA12840.2893853716145405No Hit
TTCAGTCTACATTGT12660.2853285673395703No Hit
GAGTTCTCCGGGCCT11990.2702282403160701No Hit
ATCACGGTCCTGTTT11860.26729832611748056No Hit
AATGCCGTAATTGTG11440.2578324494758834No Hit
GGACTAATTCCTGTT9920.22357499115391288No Hit
TCGTAGCCTCGGGGT9760.21996894290949495No Hit
AGCACTGCAAGAGGA9430.21253146840538292No Hit
AGTCTCCCGTAGGTG9050.2039671038248903No Hit
GGACTAATGGGTATC8310.1872891306944573No Hit
GCGTCTTTGTTCATC8110.18278157038893486No Hit
CAGTCAATTAAGTTT7470.16835737741126303No Hit
AACGGTGTTCAGTGT7050.15889150076966593No Hit
GTAATCCTGAAGATT6880.15506007450997186No Hit
CATCTGGTAAGTCGG6270.1413120155781284No Hit
GAATAGCTGTGGAGA6150.13860747939481496No Hit
TGAGCAGATGAGACT6040.13612832122677762No Hit
AACATCGTAATATGG5770.13004311481432232No Hit
ACATCCGGCCTGTCA5280.11899959206579235No Hit
ATCCAGGGGTATGCG4800.1081814473325385No Hit
GCCATAGGGTGCCAC4600.10367388702701606No Hit
ATCCAGGTGTAAGGT4580.10322313099646381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)