FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535722
Sequences flagged as poor quality0
Sequence length15
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN10876120.301760987975108No Hit
TCTAGTCTGATAGCC327306.109512023026868No Hit
CATGAACGACGCAGC213223.9800493539559696No Hit
CGTTGAATTACGTCT206893.8618910554354686No Hit
TCTTCGATCGACGCT173033.2298468235390745No Hit
GTATGCGATAAAACT165943.0975020626369645No Hit
AACAGCTACGATTTG159002.9679572614154357No Hit
CAGGAATAGAGAATT123072.2972735859270297No Hit
CATCTGGGGGGTACT122302.2829004595667155No Hit
GGCTAGATCGGATTA119202.22503462616805No Hit
CCTGATGGGCATTCT116432.173328704066661No Hit
ATAACCGGTTAGCCT110762.0674902281407146No Hit
ATCCAGGTAGACCCG108642.0279174646551756No Hit
TTACTGCCGCAGCGT99841.863653163394447No Hit
CATTGTCGCCGCTCT93921.753148088000866No Hit
GTCGAGAGGATATGG86291.6107234722486663No Hit
TATGTCCAATATAAT79491.4837919667290125No Hit
AAGGCTAGTTTTTTT74691.3941932569504334No Hit
TCGTAGCTAGAGTCG67871.2668884234733686No Hit
CGTATGAGTCTATCT54961.0259052269647317No Hit
CAACGGTGTGGTGTT54421.0158253721146415No Hit
ATCTACGTAGTGTAG49430.9226800467406603No Hit
CAATGCGATCTTCTT46670.8711607886179772No Hit
GTCACACCAGAAAGA43110.8047084121988642No Hit
CAATGCGCATACAAC38930.7266828691000182No Hit
TGCCTAAAAGTTATA36500.6813235222746126No Hit
TGTCTCATACAGCTT36070.6732969711902815No Hit
GTAGTCTGCAGAGCT31020.5790316619440681No Hit
ACCAGTGGCATACCT30510.569511799030094No Hit
TCTTCGAGGCTGGAT25980.4849530166765598No Hit
CTACAAGTAATCTTT25400.47412650591164823No Hit
ATAGGTCCATAACAG25070.4679665946143709No Hit
GCTACCAAGTCTGTC22690.42354056768249204No Hit
GTTAGGCTGCGTTAT20990.39180769130257853No Hit
GTCGAGAGCCCGGGG20320.37930120472931855No Hit
AACAGCTAATCGATG19390.36194145470971884No Hit
ACTCTTGCGTGTTTG18370.34290172888177073No Hit
TGGTCAACTAGCAGT16230.30295563743882087No Hit
GGCTAGACTCTAGCT15990.2984757019498919No Hit
TTACTGCTGCCTAAT15040.28074262397288147No Hit
CACAGATGATTGTGC13870.25890293846435275No Hit
ATGTCGCCGCACTTG12610.23538327714747576No Hit
GCATGGAAAGTAGGG12470.23276998144560052No Hit
AGTTCGCGTTTGGCA12390.23127666961595753No Hit
ACAATCGGTGCAGGT11110.20738368034166976No Hit
GCATCGTTGTTTTTT10990.2051437125972053No Hit
CGTAACTTGGGGGTG9880.18442401096090882No Hit
CGTAACTGTCTAAGT9750.18199737923773898No Hit
TGCGTATGGAACAAT9560.1784507636423369No Hit
AGATCGAGAGTAGTA8820.16463762921813926No Hit
TCTAGTCTCGCTGAT6730.12562485766871623No Hit
TACGTACCTGGGAAT6510.12151825013719801No Hit
AATGCCGCCAAACTT5720.10677179581947353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)