FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456568
Sequences flagged as poor quality0
Sequence length15-160
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG7569716.579567556201923No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA6015313.175036358220463No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA5680312.441301186241699No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA325467.12840146484204No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG241545.290340102679119No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG183124.010793572917945No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA141733.1042473410313467No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC131612.8825936114664192No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC60951.3349599621524066No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC54911.2026686057717582No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG53591.1737572497415498No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT52091.1409034360708592No Hit
CCCTGATGGCTACAATGTCTCCAGATTTGCCAGCAGTCCCCGGGGCGAGT50241.1003837325436738No Hit
TGGCTACAATGTCTCCAGATTTGCCAGCAGTCCCCGGGGCGAGTATGGCT42270.9258204692400693No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG41980.9194687319304025No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG41600.911145765800494No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG34990.7663699602249829No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG30260.662770934450071No Hit
CGATTCTCAGGGCGCCAGTTCTCTATCTCTCGCTCTGAGATGAATGTGAG28410.6222512309228856No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA27650.6056052986630688No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT24440.5352981374077903No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA22620.49543551015401865No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA22520.49324525590930596No Hit
GTTTCCCATCAGCCGCCCAAACCTATCATTCTCAACTCTGACTGTGAGCA21360.4678383066706383No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT20690.45316360323106303No Hit
TCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCC17790.38964623013439403No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT17650.3865798741917962No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA16590.3633631791978413No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG16500.36139195037759986No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG15630.34233673844859913No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC15340.3359850011389322No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA15230.3335757214697482No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC13980.30619754341083916No Hit
CTGCAGAGAGGCCTAAGGGATCTTTTTCCACCTTGGAGATCCAGCGCACA12260.26852517040178026No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC11600.25406949238667625No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC10270.224939110931997No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC9550.20916928037006532No Hit
CCTGAATGCCCCAACAGCTCTCTCTCAAACCTTCACCTACACGCCCTGCA9480.20763610239876643No Hit
TCTCCACTCTGAAGATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTAC9110.19953216169332935No Hit
GGGGTACAGTGTCTCTAGAGAGAAGTAGGAGCGCTTCTCCCTGATTCTGG8830.19339944980813373No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC8250.18069597518879993No Hit
GGGGTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG8230.18025792433985738No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC7460.16339296665556938No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG7110.15572707679907483No Hit
GCCTGATGGATCAAATTTCACTCTGTAGATCCGGTCCACAAAGCTGGAGG6960.15244169543200575No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT6360.1393001699637294No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT5810.1272537716178094No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT5730.1255015682220392No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC5060.11082686478246395No Hit
CAGACCCCAGGACCGGCAGTTCATCTTGAGTTCTAAGAAGCTCCTTCTCA4800.10513220374621086No Hit
CCCTGATGGCTACAATGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCA4750.10403707662385449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)