FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456568
Sequences flagged as poor quality0
Sequence length15
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN379328.308072401044313No Hit
CTAGACGGACAGGTT345987.57784163585709No Hit
AGATCACTAGGAGTT137283.0067810271416304No Hit
CCTGATGGACTCAAT131682.884126789437718No Hit
AGCTCAATGTTTTTC118802.602022042718719No Hit
CAGTACGTCGCGGGC118302.591070771495155No Hit
ATAACCGCATTGTTG111042.4320583133290112No Hit
ACAATCGTAATCTCT110632.4230782709256893No Hit
GGCACTTCCCGGTTT109492.3981093725359637No Hit
GTATGCGTTCTTACC107892.3630653046205605No Hit
GTAATCCTTGACTGG94102.0610292442746756No Hit
AGGAGTCACACCCTC93072.0384696255541344No Hit
GCTTCATGGTGAAGA90711.9867796253789138No Hit
ATAACCGCACTGGCT84071.8413467435299888No Hit
TGTCTCAGAGGTGAT83791.8352140316447934No Hit
TCGATACTTTGGTTT81971.7953514043910217No Hit
TCGTAGCTTGTGGCT81021.7745439890662507No Hit
GCGATTCTAGAGCGT78791.725701319409157No Hit
GAGCTGATGTTGTTT62751.3743845385572357No Hit
TACGACTGTGACGAC60651.3283891994182684No Hit
CGTGCATTGACGGCT58181.2742899195738642No Hit
ATCAGGCGGGGATTT57351.2561108093427487No Hit
AGCTCAAGGACCTTA56481.2370555974137478No Hit
CGTCAGATTGGGGAG55571.217124283786862No Hit
GCATCAGCACAAATT55401.2134008515708503No Hit
TTACTGCTTATCGTT53861.1796709362022744No Hit
ACGTGGTTTCGGGTT52991.1606157242732735No Hit
GAATCTGGGGTTGCG51641.131047291969652No Hit
ACGTGGTGAGATGGG49891.092717842687179No Hit
TCAGTGGTTTTCAAT47291.0357712323246484No Hit
TTCATGGAAAGAGGT46611.0208775034606017No Hit
ATAGGTCTTGGTGCT46081.0092691559636242No Hit
GCATACTCTCGCACT43500.952760596450036No Hit
AGATCACGGCACTGT39340.8616460198699865No Hit
TCTCGAATTCGAGTG37280.8165267824289044No Hit
TATGTCCAATCTTGT36850.8071086891766397No Hit
TCGATACCTTGCTTA34190.748847926267281No Hit
GACTGTGGAATCTTT30610.6704368243065655No Hit
GGCGATTTGTGGCGC28600.6264127139878397No Hit
GCTCATACAGTACCT25660.5620192391932856No Hit
AGTGAGCTATGGACT21640.47397101855583396No Hit
GCTGACATGCTTGGT21260.4656480524259256No Hit
CCTGATGTCACTCTG20990.45973436596520123No Hit
GGCGATTATCGGTCT20830.4562299591736609No Hit
GAGCTGACATATGTA19900.43586059469783256No Hit
ACAATCGCTTTTTTC19320.42315712007849876No Hit
TGGATGCAGTGGTCG16570.36292512834889873No Hit
GGTCTTATGGGCAAT15170.3322615689229205No Hit
GTGAGATATGGGTTT14910.3265669078866675No Hit
AGTGCGTTCATTTCC13940.30532144171295406No Hit
ACGTGGTTGATCCTG12380.2711534754954355No Hit
GCTGAATGGTGGTAG11200.24530847540782533No Hit
CTGATGGCTGAAGGT11180.24487042455888278No Hit
CTTACGGATGCAGAC10080.2207776278670428No Hit
GCATCCAGCTATTAC9970.21836834819785883No Hit
CATTGTCACTCTGTG9690.21223563631266318No Hit
GCATACTATACGGGA9050.19821800914650176No Hit
GGAATCGTTCAGCTT7900.17303008533230538No Hit
CGGATGTGTGGAGTT6330.13864309369031558No Hit
TCGAAGGGTGTAACG6070.13294843265406248No Hit
GGACATTGGGCCCTG5490.12024495803472866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)