FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499026
Sequences flagged as poor quality0
Sequence length15
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN7612515.254716187132534No Hit
CGCAATCAGTGATGT256915.148228749604229No Hit
ACGGTAGCTCAACAG241054.830409637974775No Hit
ATGACGTGGTGAATC237824.765683551558436No Hit
TGGCTATCTACGCTG194343.8943862644431353No Hit
CACAGTATATGTGGT189103.7893817155819534No Hit
ATAGCCTGATCTTGC175393.5146465314432516No Hit
ATGCTACTTTGGTTT160743.2210746534248713No Hit
TCTGGAGGTGACGGT157393.153943882683467No Hit
ACGCAGGGGCGAACC123632.4774260258984504No Hit
TGGCTATATGTTGTG112542.255193116190339No Hit
ATCGCCAGTTGTTTT108112.1664201865233474No Hit
GAATCTGGTTCTTCC94761.898899055359841No Hit
TGCCAGAGCCGTTGT91131.8261573545266179No Hit
CGTATGACGGATGTG81571.634584169963088No Hit
GCCATTCGTGTTAGT81051.6241638712211386No Hit
ATGAGCGATTCTCTT80101.605126786981039No Hit
CAACGGTGGCTTTTT70521.4131528216966651No Hit
TCTTAGGCCGCGGGT58621.1746882927943634No Hit
AACTCGTAGCTGGGT55471.1115653292614012No Hit
CGGATGTTTGCTTGG49360.9891268190434968No Hit
TCACTTGGTGATGAG47850.9588678746197593No Hit
GATCGCTTGTGTTCT42490.8514586414335125No Hit
GGTCCATGGTGTGTA41770.8370305354831211No Hit
ACGGTAGGATGCTGA41060.8228028198931518No Hit
CTGGAAGTCAGTCTA40800.8175926705221772No Hit
GGACGATCACAACCG39420.7899388007839271No Hit
CAGTCAAATGGCGGG31350.6282237799232906No Hit
GTGTCTAGCACAACC30610.613394893252055No Hit
GTCACACAGTGGGTG28310.5673051103549714No Hit
TGACGGAATTCAAAC28140.5638984742277957No Hit
AGGCTAGTAGGGGAG27170.5444606092668518No Hit
AATGCCGTGTGGTTT24630.4935614577196379No Hit
GGAATCGAGCTTCTT24160.48414311077979905No Hit
TACTGACGAGTTGTT23830.4775302288858697No Hit
GCTGATTACTCGTAT23450.46991539518982983No Hit
GTTGACTTGCGGGTT21740.4356486435576503No Hit
GCTCATAGAGGAAAA18290.36651396921202506No Hit
GCATCGTTGGACTCC15760.31581520802523316No Hit
CGTATGACGGCTGTG15300.30659725144581645No Hit
CGTATGACGGATGCG11310.22664149763739766No Hit
GAATCTGCTCACGCT9100.18235522798411305No Hit
AGCAATCAGTGATGT8010.1605126786981039No Hit
CTACGATCTCGGAAA7820.15670526185008396No Hit
TCACTTGTATCAGTT7400.14828886671235567No Hit
CTACCGAACGGGGTC5870.11762914156777401No Hit
CGTATGCCGGATGTG5150.10320103561738267No Hit
AACAGTATATGTGGT5000.10019518021105113No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)