FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462410
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA7762516.787050453061138No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG6816114.740381912155879No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA331577.1704764170325035No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC330337.143660387967388No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC310786.720875413593996No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA301206.513699963236089No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA166593.602647001578686No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA98002.1193313293397633No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA79181.7123332107869638No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC69201.4965074284725677No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC64251.3894595705110182No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG49701.0748037455937371No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG45750.9893817175234099No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC45380.9813801604636577No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG39890.8626543543608487No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG30270.6546138708072923No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA29070.6286628749378257No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG28470.6156873770030925No Hit
GTTTCCCATCAGCCGCCCAAACCTATCATTCTCAACTCTGACTGTGAGCA28410.6143898272096192No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA28090.6074695616444281No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC27970.6048744620574814No Hit
GGGGTACAGTGTCTCTAGAGAGAAGTAGGAGCGCTTCTCCCTGATTCTGG25620.5540537618131096No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA18260.3948876538137151No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT15590.33714668800415215No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT15360.3321727471291711No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA14180.30665426785752903No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG13190.28524469626521914No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG13000.281135788585887No Hit
GCAGAGAGGCTCAAAGGAGTAGACTTCACTCTCAAGATCCAGCCTGCAAA11440.24739949395558053No Hit
CTGCAGAGAGGCCTAAGGGATCTTTTTCCACCTTGGAGATCCAGCGCACA11140.2409117449882139No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT9450.20436409247204862No Hit
CGATTCTCAGGGCGCCAGTTCCCGAACAAAGATGCCCTAGTCAGGCACCA8440.1825220042819143No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA7330.1585173331026578No Hit
NNNNNNNNNNNNNNN6840.147920676455959No Hit
GGGGTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC6670.14424428537445125No Hit
CTCAGCAGAGATGCCTGATGCAACTTTAGCCACTCTGAAGATCCAGCCCT6450.13948660279838238No Hit
GATAACTTCCAATCCAGGAGGCCAGCAAGCGCATCTCCAGCCACATCCCT5690.12305097208105362No Hit
GATCCATCTCCACTCTGACTGTGAGCAACATGAGCCCTGAAGACAGCAGC5590.1208883890919314No Hit
TGGCTACAATGTCTCCAGATTAACCACAGAGGATTTCCCGCTCAGGCTGC5560.12023961419519474No Hit
GTCTCCAGATCAACCACAGAGGATTCCCCGCTCAGGCTGCTGTCGGCTGC5260.11375186522782813No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCTCTCAGGCTGCTGTCGGCTGC5200.11245431543435479No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC5140.11115676564088146No Hit
TGGATACAGTGTCTCTCGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG4850.10488527497242706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)