FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469275
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG8466218.0410207234564No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA332997.095839326620851No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC327296.974375366256459No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA325836.94326354482979No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG301856.43226253263012No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA177033.772414895317245No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA161403.4393479303180436No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC151903.236907996377391No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA147853.150604656118481No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC93271.987533961962602No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA90271.9236055617708168No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC65761.4013105322039316No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC62331.3282190613179905No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA56031.1939694209152416No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC54291.1568909488040062No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA48401.0313781897608012No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG43920.9359117788077354No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG39120.8336263385008791No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA36100.7692717489744819No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA33020.7036385914442491No Hit
CGATTCTCAGGGCGCCAGTTCTCTATCTCTCGCTCTGAGATGAATGTGAG31140.6635767939907303No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA30760.6554791966331043No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC28260.6022055298066166No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG25520.5438175909647861No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCAAA24790.5282616802514517No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA22920.48841297746523893No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG21360.45517020936551067No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT20220.43087741729263224No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG19140.4078631932235896No Hit
GTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAGC18040.384422779819935No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC17350.36971924777582443No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT17050.36332640775664593No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT13810.29428373554951787No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG12890.2746790261573704No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG12240.26082787278248365No Hit
GTTTCCCATCAGCCGCCCAAACCTATCATTCTCAACTCTGACTGTGAGCA11310.2410100687230302No Hit
GGGGTACAGTGTCTCTAGAGAGAAGTAGGAGCGCTTCTCCCTGATTCTGG11220.23909221671727668No Hit
CAGACCCCAGGACCTGGACCCAGAGCCCTAGAAAGGGAAACACTGGTTTC9860.21011134196366735No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT9560.20371850194448884No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG8710.18560545522348304No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG8300.17686857386393906No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA8200.17473762719087954No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT8160.17388524852165574No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT7770.16557455649672367No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT7400.1576900538064035No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT6870.1463960364391881No Hit
CTGCAGAGAGGCCTAAGGGATCTTTTTCCACCTTGGAGATCCAGCGCACA6520.13893772308347985No Hit
GATAACTTCCAATCCAGGAGGCCAGCAAGCGCATCTCCAGCCACATCCCT6500.13851153374886793No Hit
NNNNNNNNNNNNNNN6440.13723296574503224No Hit
TGGCTACAATGTCTCCAGATTAACCACAGAGGATTTCCCGCTCAGGCTGC6410.13659368174311437No Hit
TCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCC6150.13105322039315964No Hit
CGATTCTCAGGGCGCCAGTTCCCGAACAAAGATGCCCTAGTCAGGCACCA6120.13041393639124182No Hit
CAGACCCCAGGACCGGCAGTTCATCTTGAGTTCTAAGAAGCTCCTTCTCA5930.12636513771242874No Hit
GGGGTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC5840.1244472857066752No Hit
TCTCAGCTCAACAGTTCAGTGACTTGCACTCTGAACTAAACCTGAGCTCT5640.12018539236055617No Hit
CTCAGCAGAGATGCCTGATGGTTCAAGTAGAGGTATGTTCTAACCTTTAG5620.11975920302594427No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC5490.11698897235096692No Hit
GCTAAGATGCCTAATGCATCATTCTTCACTCTGAAGATCCAGCCCTCAGA5290.1127270790048479No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)