FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265919
Sequences flagged as poor quality0
Sequence length35-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA5739821.584768294104595No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA163696.1556338584305745No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG153925.788228746347572No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA133175.007915944328912No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG55882.1013917771953112No Hit
GTTTCCCATCAGCCGCCCAAACCCACTCCACCTTACTACCAGACAACCTT49451.8595888221601315No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA47911.8016764503476623No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA44521.6741940214877464No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA38701.455330382560103No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA35401.3312324429619544No Hit
CAGACCCCAGGACCCCCTCTCAGGTCTAGAAGATCCAGTTGGGCAGTGTT33951.276704560411253No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG31131.170657230209199No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA28381.0672422805440755No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG24610.9254697859122516No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG21400.8047563355758708No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT16970.6381642530244173No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA15700.5904053489972511No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC15280.5746110657756686No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC15060.5663378698024586No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA14730.5539280758426438No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA12850.4832298557079412No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12810.4817256382582666No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGCGCA12500.4700679530232891No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC11860.4460004738284966No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC10300.3873359932911902No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG9120.3429615785257917No Hit
GTTTCCCATCAGCCGCCCACACCTAACATTCTCAACTCTGACTGTGAGCA8540.3211504255055111No Hit
GTTTCACATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA8320.3128772295323012No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG8150.3064843053711845No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGCCTGTGAGCA7210.2711351953038331No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA6780.25496485771983196No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC6620.2489479879211339No Hit
GGTACAGCGTCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGC6340.23841846577341222No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA5560.20908622550475897No Hit
GTTTCCCATCAGCAGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA5270.19818064899461865No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGCTGAATGTGAG5190.1951722140952696No Hit
GTCTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA4800.180506093960943No Hit
CCTGAATGCCCCAACAGCCGCCCAAACCTAACATTCTCAACTCTGACTGT4790.18013003959852436No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT4650.17486527852466355No Hit
GATCCATCTCCACTCTGACTGTGAGCAACATGAGCCCTGAAGACAGCAGC4520.16997657181322132No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT4450.1673441912762909No Hit
ATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC4210.15831888657824375No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGACTGTGAG4030.1515499080547084No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA3670.13801195100763766No Hit
GTTTCCCATCAGCCGCCCAAACCTAACCTTCTCAACTCTGACTGTGAGCA3580.13462746174586998No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT3550.13349929865861407No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC3430.1289866463095905No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCCACTCTGACTGTGAGCA3420.1286105919471719No Hit
TCTCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG3420.1286105919471719No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCC3340.12560215704782285No Hit
GTTTCCCATCAGCCGCCCAAACCTACCATTCTCAACTCTGACTGTGAGCA3290.12372188523572968No Hit
GTTTCCCATCAGCCGCCCAAACCACATGCCTCAAAAATTCAGAGTGAAAT3280.12334583087331105No Hit
GTTTCCCATCAGCCGCCCAACCCTAACATTCTCAACTCTGACTGTGAGCA3160.11883317852428747No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG3160.11883317852428747No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG3130.11770501543703157No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGCATGTGAG3120.11732896107461295No Hit
GTTTCCCATCAGCCGCACAAACCTAACATTCTCAACTCTGACTGTGAGCA3080.11582474362493841No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC3070.11544868926251979No Hit
GTTTACCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA3060.11507263490010117No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGCG3010.11319236308800801No Hit
CCTGAATGCCCCAACAGCCTTCCCAGCCCCAGCGGACCCTGCTCCAGCAT2980.11206420000075211No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGCGATGAATGTGAG2870.10792760201414717No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG2750.10341494966512359No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG2700.10153467785303043No Hit
GTTTCCCATCAGCCACCCAAACCTAACATTCTCAACTCTGACTGTGAGCA2700.10153467785303043No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTATGACTGTGAGCA2660.1000304604033559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)