FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250105
Sequences flagged as poor quality0
Sequence length35-160
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA229649.181743667659582No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA123014.918334299594171No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC110814.4305391735471105No Hit
GTTTCCCATCAGCCGCCCAAACCCACTCCACCTTACTACCAGACAACCTT100524.019111972971352No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA88793.5501089542392195No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG85273.4093680654125267No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA82743.308210551568341No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG37231.4885747985845945No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG35931.4365966294156454No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG29931.1966973870974191No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT29101.1635113252433977No Hit
CAGACCCCAGGACCCCCTCTCAGGTCTAGAAGATCCAGTTGGGCAGTGTT24660.9859858859279104No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG24390.9751904200235901No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA20720.8284520501389416No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC18730.7488854681033966No Hit
TCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCC18390.7352911777053638No Hit
TCTCACCTAAATCTCCAGACAACCTTAACCAAACCATTTACCCAAATAAA16950.6777153595489894No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC16450.6577237560224706No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT15750.6297355110853441No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG15220.6085444113472341No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG14780.5909518002438976No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA14250.5697607005057875No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA13940.5573659063193459No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC13440.537374302792827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13220.5285779972411587No Hit
TCTCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG12240.48939445432918177No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA11340.4534095679814478No Hit
GGTACAGCGTCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGC11270.45061074348773517No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT11120.4446132624297795No Hit
CAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCT11050.44181443793606684No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT8820.35265188620779275No Hit
ATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC8780.3510525579256712No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC6820.2726854721017173No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC6500.2598908458447452No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTG6120.24469722716459089No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC5910.23630075368345294No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT5730.22910377641390617No Hit
GTTTCCCATCAGCCGCCCAAACCATTTACCCAAATAAAGTATAGGCGATA5420.2167089822274645No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC5400.21590931808640373No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC4920.19671737870094558No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC4780.19111972971352031No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC4450.17792527138601788No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA4330.17312728653965334No Hit
CCTGAATGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAA4290.17152795825753184No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG4220.1687291337638192No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG3830.15313568301313446No Hit
CCTGAATGCCCCAACAGCTCTCTCTTCAACCTTCACCTACACGCCCTGCA3710.14833769816676995No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC3680.14713820195517882No Hit
TCTCAGCTCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCT3580.14313988124987506No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAACCCTTCACCTACACGCCCTGCA3270.13074508706343335No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA3250.1299454229223726No Hit
GTTTCCCATCAGCCGCCCAAACCCACCTTCAGGTCCTACCTCCTGCCACC3140.12554727014653846No Hit
CCTGAATGCCCCAACAGCACCTCCTACCTCGGCTCCAAGATCATCAAAGC3020.12074928530017393No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG2840.11355230803062714No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGCGCTCTC2820.11275264388956638No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG2790.11155314767797525No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA2780.11115331560744487No Hit
CCTGAATGCCCCAACAGCTCTCTCTTACACCTTCACCTACACGCCCTGCA2630.10515583454948922No Hit
CTCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG2580.10315667419683733No Hit
GTTTCCCATCATCCTAGCCCCAAACCCACTCCACCTTACTACCAGACAAC2580.10315667419683733No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCCCCTACACGCCCTGCA2540.10155734591471581No Hit
GATCATCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCC2530.10115751384418543No Hit
AAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC2510.10035784970312468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)