FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234206
Sequences flagged as poor quality0
Sequence length35-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG208228.890463950539269No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA156046.662510781107231No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC90613.8688163411697394No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA69642.9734507228679026No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG66272.8295603016148174No Hit
GTTTCCCATCAGCCGCCCAAACCCACTCCACCTTACTACCAGACAACCTT64752.764660170960607No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA62152.653646789578405No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA41531.7732252803087882No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG39581.6899652442721365No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC34081.4551292451944016No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG32931.406027172659966No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG31871.36076787101953No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC25631.094335755702245No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA23861.0187612614535921No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA22650.9670973416564905No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT22150.9457486144676055No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG20910.8928037710391707No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA17120.7309804189474223No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA15570.6647993646618788No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG14170.6050229285330009No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC13270.5665952195930078No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC13140.5610445505238978No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA12750.5443925433165675No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG12420.5303023833719034No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT11660.4978523180447981No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT10540.4500311691416957No Hit
ATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC9950.4248396710588115No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC9780.41758110381459057No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9620.41074951111414737No Hit
TCTCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG9520.40647976567637034No Hit
GGTACAGCGTCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGC9370.40007514751970485No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC8560.36549020947371114No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC8440.36036651494837874No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT8290.35396189679171325No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA8210.3505461004414917No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT6610.2822301734370597No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTG6540.27924135163061575No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA6490.2771064789117273No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA5790.24721826084728832No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT5130.2190379409579601No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGACTGTGAG4730.20195895920685208No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGCTGAATGTGAG4610.19683526468151968No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT4420.1887227483497434No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT4020.1716437665986354No Hit
CCTGAATGCCCCAACAGCTCCCTTAACCCCATGTGAGTTGCCATTTATCA3990.17036284296730228No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC3940.1682279702484138No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC3800.162250326635526No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG3640.1554187339350828No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGCG3600.153710835759972No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC3540.1511489884973058No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGCGCA3230.13791277764019708No Hit
GTTTCACATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA3050.13022723585219848No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC3040.1298002613084208No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGCGATGAATGTGAG3000.12809236313331No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGCATGTGAG2920.12467656678308839No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG2860.12211471952042219No Hit
CAGACCCCAGGACCTCTTCTGGAGATGGAAGCTTGTTGAAAACCCAGACT2850.12168774497664449No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACA2800.119552872257756No Hit
TCTCAGCTCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCT2770.1182719486264229No Hit
TCTCACCTAAATCTCCAGACAACCTTAACCAAACCATTTACCCAAATAAA2730.11656405045131209No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG2670.11400220318864589No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG2640.11272127955731279No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA2570.10973245775086889No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGCGCTCTC2550.1088785086633135No Hit
CCTGAATGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAA2530.1080245595757581No Hit
CCTGAATGCCCCAACAGCACGGCGTGGCCTCTCAGCCTCGAGCCTGATCC2410.10290086505042569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)