FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258355
Sequences flagged as poor quality0
Sequence length35-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA204937.932108919897041No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA111814.3277660583305915No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG105004.0641752627199015No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC97063.7568461999961293No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC89493.4638385167695613No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA80873.130189080915794No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA73362.8395037835536376No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA68092.6355208917961717No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG67322.605716939869559No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA53602.0746647055408256No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG48301.8695206208511543No Hit
GTTTCCCATCAGCCGCCCAAACCCACTCCACCTTACTACCAGACAACCTT47761.848619148071452No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG44371.7174043467322093No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT33151.283118190087283No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA29821.1542257746124518No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC26611.0299781308664435No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG23860.9235354454142556No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA22100.8554121267248552No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA21310.8248340461767723No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC20430.7907723868320721No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA19460.753227148690755No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC19190.7427764123009037No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA18150.70252172398444No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG18030.6978769522556173No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT16120.6239476689051886No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT11270.4362214781986027No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC9890.38280660331714117No Hit
GGTACAGCGTCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGC9200.35609916587641033No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA8800.34061659344700124No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG8250.31932805635656364No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG8160.3158444775599466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8120.31429622031700566No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG7770.30074896944127266No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG7390.286040525633334No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC6860.26552611716436686No Hit
ATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC6830.2643649242321612No Hit
CAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCT6650.25739776663892705No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC6430.24888235180275203No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA6330.24501170869539973No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG6200.23997987265584178No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACA5810.22488436453716784No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT5650.21869133556540418No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATCCAGCGCACA5310.2055311490004064No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGCGCA5270.2039828917574655No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC4830.18695206208511544No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT4780.1850167405314393No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG4590.17766251862746996No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA4560.17650132569526428No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC4490.17379187552011768No Hit
TCTCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG4350.16837297516982447No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC4330.16759884654835402No Hit
GGGGAACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG4080.1579222387799733No Hit
GTTTCACATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA4010.1552127886048267No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA3650.14127847341835847No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT3330.12889241547483116No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC3260.12618296529968456No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT3090.11960287201718567No Hit
GTTTCCCATCAGCCGCCCACACCTAACATTCTCAACTCTGACTGTGAGCA3060.11844167908497998No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGCCTGTGAGCA2960.11457103597762767No Hit
TCTCAGCTCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCT2850.11031332855954018No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC2800.10837800700586402No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT2710.10489442820924696No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGCGCTCTC2690.1041202995877765No Hit
CTCAGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA2660.10295910665557083No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT2590.10024965648042423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)