FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271234
Sequences flagged as poor quality0
Sequence length35-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC218408.052087865090659No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG177746.553013265298597No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA123604.5569508247491095No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA112964.164669621065205No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA106483.9257615195735047No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA100183.693489754234351No Hit
GTTTCCCATCAGCCGCCCAAACCCACTCCACCTTACTACCAGACAACCTT89323.293097473030667No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT74592.7500239645472173No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG63832.3533185367616154No Hit
GCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTACGAA53231.9625120744449442No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC34161.2594291276167442No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC29181.0758238273962704No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC29091.0725056593199966No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG26940.9932383108312379No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA21450.7908300581785469No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC20640.7609665454920843No Hit
GGTACAGCGTCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGC18260.6732194341417373No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT17890.6595780764948348No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG13320.4910888752884963No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13300.4903515046048799No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA12160.4483213756387473No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT11780.4343113326500365No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG11550.4258315697884483No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC11080.4085033587234639No Hit
ATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC8670.3196501913476924No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC7700.2838877131922989No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATC7620.28093823045783345No Hit
CAGACCCCAGGACCGGCCCGTGGTACCCAGCAGGTGGCACTGCAGCTCCC7340.2706150408872044No Hit
TCTCAGCTCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCT7130.2628726487092326No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT6590.2429636402515909No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA6130.22600411452841457No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGCGCTCTC6080.22416068781937365No Hit
CTCCGCACAACAGTTCCCTGACTTAAGACTCATCCAGTATGATTGCGGTG6070.2237920024775655No Hit
TCTCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG6020.2219485757685246No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT5990.22084251974310004No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT5720.2108880155142792No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG5650.20830721812162192No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTACACCTGAGCTCTC5640.20793853277981375No Hit
TCTCAGCTCAACAGTTCAGTGACTTGCACTCTGAACTAAACCTGAGCTCT5440.20056482594365013No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA5310.1957719165001438No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG5050.1861860976131311No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC4900.1806558174860084No Hit
CGATTCTCAGGGCGCCTTCTCCCCTCAGGGCTCCACTAGCTGAGGATGGA4890.1802871321442002No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT4770.17586290804250204No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC4530.1670144598391057No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT4020.14821150740688852No Hit
GGAACGATTTTCTGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAA3960.14599939535603945No Hit
CTCAGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC3610.13309540839275313No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA3540.13051461100009584No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC3470.1279338136074386No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC3450.12719644292382223No Hit
CAGACCCCAGGACCGCAGCCTCCTCTCAGCAACCACGGGAGAAAGCTGCT3440.12682775758201406No Hit
CCTGAATGCCCCAACAGCACGGCGTGGCCTCTCAGCCTCGAGCCTGATCC3150.11613588266957682No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGCCAG3070.11318639993511138No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC3040.11208034390968682No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC2990.11023691720064593No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG2940.10839349049160504No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG2800.1032318957062905No Hit
GGACACGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG2780.10249452502267414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)