FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566663
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG9976817.606231569733684No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC6140710.836599530938141No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG280634.95232616211046No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG237764.195791855123768No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG214603.7870833281862417No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG203293.5874938014304796No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC139422.4603688612102785No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA117782.0784840372496527No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT114492.02042483804307No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA85781.5137745008938295No Hit
GGTACAGCGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAG82361.4534211691958006No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA76971.3583029066658667No Hit
CCCGAATGGCTACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC72481.2790670998459404No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT69801.23177267617614No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC48970.8641820623545212No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA46410.8190052994460552No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT44770.7900639357078193No Hit
TGGCTACAATGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG44030.7770050276795909No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG41900.7394165491659064No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT39310.6937103710671069No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA38300.6758867263258762No Hit
TGGATACAGTGTCTCTCGACAGGCATAGGCTAAATTCTCCCTGTCCCTAG37210.656651307743756No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC37200.6564748360136448No Hit
CAGATGGCTATAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACT25810.45547353541699387No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC24520.43270868223264974No Hit
CCCCGATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTC23930.42229685015608925No Hit
CAGATGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACT23600.41647328306241976No Hit
GGTACAGCGTCTCTCGGCAGGCACAGGCTAAATTCTCCCTGTCCCTAGAG22520.3974143362104107No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTG19740.3483551952394986No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC19610.3460610627480531No Hit
GGGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTT19240.33953160873393884No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG18870.33300215471982464No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATCCAGCGCACA18780.3314139091488239No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT18570.3277080028164888No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT17640.3112961319161477No Hit
TACAAAGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAGTC16620.2932960154448058No Hit
GGACAAGTTTCTCATCACCCAACTAAAAATATTAAACACAAACTACCACC16610.2931195437146946No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG16300.2876489200812476No Hit
GGGGTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG15510.2737076534024632No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACA14470.25535459347089895No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA14410.2542957630902318No Hit
CAGATGGCTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCC14130.2493545546471183No Hit
TGGCTACAATGTCTCCAGATCAATCACAGAGGATTTCCCGCTCAGGCTGC12460.21988377571854878No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC12360.2181190584174368No Hit
CTCTGAGCTGAATGTGAACGCCTTGTGGCTGGGGGACTCGGCCCTCTATC12350.21794258668732563No Hit
GGACAAGTTTCTCATCAACAACCGACTAATCACCACCCAACAATGACTAA12060.212824906514101No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCACTTCC11470.2024130744375405No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC11280.19906011156542777No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT10670.18829533602864487No Hit
CCCTGATGGCTACAATGTCTCCAGATCAATCACAGAGGATTTCCCGCTCA10400.1835305993156426No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT10130.17876586260264038No Hit
GGGGTACAGTGTCTCTAGAGAGAAGTAGGAGCGCTTCTCCCTGATTCTGG9870.1741775976197493No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC9530.16817755879596868No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA8920.15741278325918578No Hit
GGGGTACAGTGTCTCTCGACAGGCATAGGCTAAATTCTCCCTGTCCCTAG8810.15547159422796264No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC8320.14682447945251412No Hit
GGGGTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGT8170.14417740350084618No Hit
GGACAAGTTTCTCATCAACCATGCATGCCTGACCTTGTCCACTCTGACAG7680.13553028872539763No Hit
CGATTCTCAGGGCGCCAGTTCTCTATCTCTCGCTCTGAGATGAATGTGAG7340.12953024990161702No Hit
ATTTCTCATATGATGTTAAAAATATTAAACACAAACTACCACCTACCTCC7230.12758906087039387No Hit
GATCCATCTCCACTCTGACGAAAACAGCAGACGGAAAAGTACTGACCAGC7130.1258243435692819No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA7090.12511845664883714No Hit
CCTCATCTTTAATGATGAACTGTCGCCAAGTCAGCTGAAGTCATTGGCAA6980.123177267617614No Hit
GTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAGC6880.12141255031650204No Hit
GTCTCTAGATTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCAT6720.11858900263472293No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT6660.11753017225405576No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC6530.11523603976261022No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT6410.1131183790012759No Hit
CCCTGATGGCTACAATGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCA6140.10835364228827364No Hit
GTCTCTAGATTAAACACAGAGGACCTCCCCCTCACTCTGTAGTCTGCTGC6020.10623598152693929No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC5960.10517715114627213No Hit
CCCTGATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCTC5890.10394184903549375No Hit
GGACAAATATCTCATCAACAACCGACTAATCACCACCCAACAATGACTAA5670.10005947097304747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)