FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460295
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC5192411.280591794392727No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA378468.22211842405414No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC313726.815629107420242No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA283406.156921104943569No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG260575.660934835268686No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA250545.443031099620895No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC193464.202956799443835No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG167323.635060124485384No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA150743.2748563421284174No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA96922.105606187336382No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC87341.8974787907754809No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG75001.6293898478149882No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT70681.5355369925808449No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG62901.3665149523675035No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG62671.3615181568342043No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA58491.2707068293159822No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC53111.1538252642327202No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC51691.12297548311409No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA41760.9072442672633855No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA36180.7860176625859503No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA28520.6196026461291129No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT24310.5281395626717649No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT24270.5272705547529302No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG22560.49012046622274846No Hit
GGTACAGCGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAG20750.4507978578954801No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG20080.43624197525499947No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA19950.43341769951878684No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT19480.4232068564724796No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT17330.3764976808351166No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC16730.36346256205259675No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG13860.3011112438762098No Hit
GTTTCCCATCAGCCGCCCAAACCTATCATTCTCAACTCTGACTGTGAGCA13800.29980773199795785No Hit
GGGGTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC12910.28047230580388666No Hit
TGGCTACAATGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG11920.2589643598127288No Hit
CCCGAATGGCTACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC10500.22811457869409835No Hit
CAACAGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCG10030.21790373564779109No Hit
TGGATACAGTGTCTCTCGACAGGCATAGGCTAAATTCTCCCTGTCCCTAG9450.20530312082468855No Hit
GGGGTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG9060.1968302936160506No Hit
GGACAAGTTTCTCATCACCCAACTAAAAATATTAAACACAAACTACCACC8620.1872712065088693No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATCCAGCGCACA8040.17467059168576674No Hit
GTGTCTCCAGAGCAAACACAGATGATTTCCCGCTCAGGCTGCTGTCGGCT7810.16967379615246744No Hit
GGGGTACAGTGTCTCTAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG7350.15968020508586883No Hit
GGCTACAATGTATCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCT7270.15794218924819953No Hit
TGGCTACAATGTCTCCAGATCAACCTCAGAGGATTTCCCGCTCAGGCTGC7220.1568559293496562No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC7050.15316264569460888No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA6820.1481658501613096No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT6820.1481658501613096No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCTCTCAGGCTGCTGTCGGCTGC6700.1455588264048056No Hit
CTCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA6660.14468981848597096No Hit
TGGCTACAATGTCTCCAGATTAAACTAACGGGAGTTCTCGCTCAGGCTGG6650.14447256650626228No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT6570.14273455066859297No Hit
GGACAAGTTTCTCATCAACAACCGACTAATCACCACCCAACAATGACTAA6420.139475770972963No Hit
GGGGTACAGTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC6330.137520503155585No Hit
GTCTCCAGATCAACCACAGAGGATTCCCCGCTCAGGCTGCTGTCGGCTGC6310.13708599919616768No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC5830.12665790417015177No Hit
TGGATACAGTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC5770.12535439229189976No Hit
GGGGTACAGTGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG5760.1251371403121911No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACA5690.12361637645423044No Hit
GGTACAGCGTCTCTCGGCAGGCACAGGCTAAATTCTCCCTGTCCCTAGAG5510.11970584081947447No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA5280.11470904528617518No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC4720.10254293442248992No Hit
GTTGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGGAGTCGGC4620.10037041462540328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)