FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500469
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG20766641.494278366891855No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG7172414.331357186958632No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG460619.203567054103251No Hit
CAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCT248634.967940072212265No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT67731.3533305759197871No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT58281.1645076917851056No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG55901.1169522987437783No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC51241.0238396384191628No Hit
CAGACCCCAGGACCGGCTAATTTTTGTATTTTTTAGTAGAGACAGTGTTT49370.9864746867438342No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA46680.9327251038525863No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC42100.841210944134402No Hit
TCTCCACTCTGAAGATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTAC37840.7560907868419423No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA33020.6597811253044644No Hit
NNNNNNNNNNNNNNN30650.6124255448389411No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG27870.556877648765458No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG24020.4799498070809581No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGTTCCACAAAGCTGGAGG20570.41101446842861394No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT20470.40901634267057496No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT19770.39502946236430225No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG14290.2855321708237673No Hit
CGGTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG12180.24337171732914525No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG11240.22458933520357904No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT10040.2006118261071115No Hit
GATCCATCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCC9820.19621594943942583No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT9230.18442700746699595No Hit
TCTCCACTCTGACGATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTAC8660.1730376906461739No Hit
GTTGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCTGC8490.16964087685750764No Hit
CAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGCGTCT7570.15125811988354923No Hit
TGGCTACAATGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGG6970.13926936533531548No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGCG6080.12148604608876873No Hit
CAGACCCCAGGACCTACTCGCCCCATCTCCTCTTCACCCCCCTCTTCCCA5920.1182890448759064No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT5720.11429279335982848No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGCTGAATGTGAG5660.11309391790500511No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGACTGTGAG5540.11069616699535835No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA5500.10989691669214276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)