FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436659
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG13230930.300302982418774No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA10745024.607302265612297No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT7299716.71716373646255No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG300296.876990970070467No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG83661.9159115007362726No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA57491.3165880011633793No Hit
CAGACCCCAGGACCCTCACAGCATCTGAGCTGGTAGGAAGAACTGTCTGG32990.7555094478758023No Hit
NNNNNNNNNNNNNNN28290.647873970306349No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG18100.41451109446959755No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG16700.3824494628531646No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA8920.2042783957275586No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT7450.17061368253030396No Hit
CGGTTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG7220.16534641447903284No Hit
GATCCATCTCCACTCTGACTGTGAGCAACATGAGCCCTGAAGACAGCAGC6180.14152920242111122No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT6080.13923908587708028No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGCG5310.12160518848804217No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGACTGTGAG5240.1200021069072205No Hit
GTCTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA5140.11771199036318958No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGCTGAATGTGAG4650.10649041929743804No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGCGATGAATGTGAG4580.1048873377166164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)