FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436659
Sequences flagged as poor quality0
Sequence length15
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTATACATATGT10166823.283156879853617No Hit
GTCGAGATCAAGGAA6583415.076753255973196No Hit
TCCTGACTTTCCGCT5604012.833813112749306No Hit
TTCCTGATGGGGTGG5571112.75846827845069No Hit
CGCATTGCTTGTTAT343867.8747947483047405No Hit
GCTTAAGATGGATGT166883.8217464886788086No Hit
NNNNNNNNNNNNNNN85811.9651490064329373No Hit
TTCCTGATGGGGGGG15520.3554260876335997No Hit
GTCCTGATGGGGTGG12760.2922188710183461No Hit
CTCGATCGGGGATGC11470.2626763676003472No Hit
TTCCTGATGGGGCGG10840.24824863337295236No Hit
GCCTGACTTTCCGCT10670.24435543524809977No Hit
GTCGCGATCAAGGAA9880.22626351455025548No Hit
GTCGAGATCAAGGCA9680.2216832814621936No Hit
TTCCTGCTGGGGTGG8940.20473641903636475No Hit
CGGTTATACATCTGT8230.1884765915737452No Hit
TTCCTGATGGGGAGG7390.16923961260388543No Hit
GTCGTGATCAAGGAA6920.15847606484694005No Hit
GGGTTATACATATGT6680.15297978514126584No Hit
CGGCTATACATATGT6670.15275077348686275No Hit
GTCGAGATCAAGGAC6480.148399552053204No Hit
GGAGACTCATTTAGT5760.13191071293618134No Hit
CGGGTATACATATGT5690.13030763135535967No Hit
CGGTTATCCATATGT5650.12939158473774728No Hit
AGGTTATACATATGT5540.12687245653931328No Hit
CGGTTATACCTATGT5360.12275024676005762No Hit
CGGTTCTACATATGT5070.11610890878236794No Hit
GTCGAGATCAAGGTA4860.11129966403990298No Hit
TCCTGACTTTCAGCT4700.10763547756945352No Hit
TTCATGATGGGGTGG4570.1046583260622133No Hit
CGGATATACATATGT4510.10328425613579476No Hit
TCCTGACTTTACGCT4500.10305524448139165No Hit
TCCTGCCTTTCCGCT4450.1019101862093762No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)