FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474078
Sequences flagged as poor quality0
Sequence length15
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN5181810.930268858711013No Hit
GAATCTGAGTGTCTT220764.656617687384776No Hit
GAATCTGTCTCTTTT183583.8723585570306995No Hit
AGTCGACTTAGATGA115072.427237711937698No Hit
CTAAGCGTTGAGGGC105542.226215939149254No Hit
CATCTGGCTTTATCG105332.2217862883322996No Hit
GCATCGTTGACTGAT97002.046076805926451No Hit
CGCTTGAAGTTAGTT88981.8769063318694392No Hit
TAACAGCACTGGTCG75691.5965727158821965No Hit
CCTGACTACATGCCT71961.517893679942963No Hit
TGCCAGACATCTGCC71031.4982766548964517No Hit
TCGACTCGGCACCAG66151.3953400073405642No Hit
CCTGACTAGTTGCTC65431.380152633111007No Hit
ATGCTACCGCGGGCC58531.2346069634110843No Hit
CGTATGAATTTGTTC56181.1850370614118353No Hit
TCACTGAGTTCTCCG55651.1738574664928556No Hit
CTGACTGGGCCTGGT55031.1607794497951816No Hit
TGCACTCGGTAATCC54211.1434827180337412No Hit
TATCGCGATGTTCCG53931.1375765169444692No Hit
CGATGATACTGTGGG52361.104459603693907No Hit
GCTCATAGAGATGGT51691.0903269082302913No Hit
ATGTGCCTAAGGTTG50401.0631161960690014No Hit
CTAAGCGAACCACGT49031.034217997882205No Hit
TGAAGCTTTACTGCA48651.02620243926105No Hit
ATGTGCCTTTCATCT47380.9994135986061365No Hit
ATGCCAAATTCATGT45300.9555389619429713No Hit
CGTTGAAATCCCGGG45130.9519530541387704No Hit
TCAGCCTACACCCTC44850.9460468530494983No Hit
TGCTGAGTGGGGTTT43990.9279063782753049No Hit
CTTAGTGGTGGGGTT43940.9268516995093635No Hit
TCGATACTACTCATT42770.902172216386333No Hit
GGACTTCGGCCTTAT40940.8635709735528753No Hit
AGATCACTGGCCACA39650.8363602613915854No Hit
CCATGGTTACTTGGT39360.8302431245491247No Hit
CATCCGTTGCTAGCC39000.8226494374343462No Hit
TCACTGAATGCGGGT36580.7716029851627791No Hit
GCTACTCGGGGGGTA35840.7559937394268454No Hit
CGTCAGAGGGTGCGT34740.732790806576133No Hit
GATGTACTATAACGC33780.7125409742700569No Hit
ATGGCTCAGTTGTTT33740.7116972312573036No Hit
CGATATCTGCTAGTG33660.7100097452317973No Hit
CTACGTAGGTGTTTT30870.6511586700922634No Hit
AACCTTGGGGGTTAT30200.6370259746286476No Hit
TGGATGCGTTGACTA29730.6271119942287978No Hit
CGCAATCCATGGGGG28980.6112918127396757No Hit
GATGTACGTGTGCTT28710.6055965474035918No Hit
TCTCGAATGATCTTG27910.5887216871485283No Hit
CTACGTAACGTCTTT26580.5606672319744852No Hit
ATCCAGGGACCGGGT26480.5585578744426023No Hit
ATGCCAAGCATCGTG25890.546112665004493No Hit
CATTGTCCTGTCTCT24610.5191128885963913No Hit
GTCAGTGGCCCATTT22770.4803007100097453No Hit
CTACCGACGAATCAC21980.46363678550787No Hit
GGAACCTTCCCCGAT21690.45751964866540945No Hit
CGTCAGAAGGGGCGA21350.4503478330570075No Hit
CTACGTACCAAATGT20000.4218715063765878No Hit
ATGGCTCCGCGTGGG17730.3739890904028451No Hit
TCACTGAGTAGTTTT16790.35416112960314544No Hit
TACGACTAGTGTTAC16450.3469893139947435No Hit
ATCGCCATATATACC16190.34150498441184785No Hit
CATGGATTTCGATAA15770.3326456827779395No Hit
CTACAAGGCCTGGAA14980.3159817582760643No Hit
AATCACGCGGGGTGC14190.299317833774189No Hit
CTACAAGTTTTGAGA13220.2788570657149245No Hit
TCACAGTTATGCGGT13180.2780133227021714No Hit
ACAGGTAGAAGTCAC13030.27484928640434697No Hit
GAGACTATTTAGTAT12990.2740055433915938No Hit
GCTCTAAGGCCTCCG12950.27316180037884064No Hit
TAGAGCCTTATTCTC12000.2531229038259527No Hit
ATGGCTCAGGACTTT11150.2351933648049477No Hit
CAACTGACCGCTAGC10800.22781061344335743No Hit
CTACAAGGGTATGTG10770.22717780618379252No Hit
ACTCGGTAATTTCCG10650.22464657714553302No Hit
CATGGATAGCATTAG10420.21979505482220227No Hit
AGTGAGCGCTGGGAT10410.21958411906901396No Hit
ACAGGTAGTAACTGT10180.21473259674568318No Hit
GCGACATGGTTTAGG9990.2107248174351056No Hit
TCGAAGGATGAGTGC8280.17465480363990735No Hit
ACGCTATTACACACG7620.16073304392947996No Hit
AGGTTCGCTTCGGTT7560.1594674294103502No Hit
CAAGGTTATCGGGCA6530.13774104683195593No Hit
TCGAAGGTTGCATTC6070.1280380021852944No Hit
TCCATGCACCGGACG5870.12381928712152852No Hit
GAGCTTCGCAGCATG5500.11601466425356165No Hit
GCATCTGAGTGTCTT5120.10799910563240647No Hit
TATAGGCTATAATAG4770.10061635427081618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)