FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477883
Sequences flagged as poor quality0
Sequence length15
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN5638711.799331635567702No Hit
GTCCAAGGAGGTACA258985.419318117614562No Hit
GTCGAGACCTCTGGA217174.544417775899122No Hit
CTTAAGCAGCAGTGT172903.6180403990097996No Hit
GTCCAAGTGAATGTT158643.3196409999937226No Hit
GACGCTAGCGGTTAC132192.7661582437542243No Hit
CGCAATCAGAGGTAT131512.7519288193972162No Hit
TGGCTATGGGGCTTT106722.233182599088061No Hit
CGAGTAGTTCTGGCC103152.1584781212137703No Hit
AGCTCAAGTATGAAT89461.872006327908714No Hit
CGAGTAGAAAGATAC89321.8690767405410948No Hit
ATGCGCATAATTGTC78701.6468466130831187No Hit
TCTAACGATGATGTT78291.638267107220805No Hit
TTAGGCATCGTTTTA76351.5976713965552238No Hit
TGGACACATATGTAG74351.555820148446377No Hit
GCAACTAGTCACCGG73821.5447295676975326No Hit
GGTCCATATGAACTC73661.541381467848825No Hit
ACTACTGGTTGAATT68081.4246164856251426No Hit
GGTCTAGTAATTTCT67861.4200128483331693No Hit
CCTGATGAATCCCAC63251.3235457214422777No Hit
CTAAGCGGAGGTGTG58761.2295896694379167No Hit
CGTATGAGCTGGTGT57101.194853133507574No Hit
ACTACTGTGCTAAGT56771.1879476775696143No Hit
GTTCAACGTATCTAT55631.1640924661475716No Hit
GCTGAATCGATCGGC55401.1592795726150544No Hit
TGGCTATCGCGACCC54411.1385632048011751No Hit
GGAACCTTTGGTTTC51931.0866676571462053No Hit
ACTACTGTTGTTTTT48321.011126154309737No Hit
TCGTGAGTTTAGGAG46410.9711582123657883No Hit
GGAGACTGAGCTAAT36950.7732018088109432No Hit
GATTCAGGGATGCGA34520.7223525423586945No Hit
AGTGCGTTTGGGCTG31190.6526702142574647No Hit
TCGTGAGTCTTTTCT27570.5769194551804522No Hit
GTGCTATCTACAGTT27400.5733620990912002No Hit
CGACTCGTCTGTTTT27180.568758461799227No Hit
TGGACACAGGTGATG26480.5541105249611307No Hit
TTCCTGAGCCAAATC26340.5511809375935114No Hit
TGATGACGAGTCAGT24780.5185369640686109No Hit
CTATCGTAAGTTTCT23980.5017964648250722No Hit
CATCTGGCGCTATGG23260.48673001550588746No Hit
AGGCTAGGTGCAGAC23220.4858929905437105No Hit
TTACTGCTGAACTAC22530.4714543099461584No Hit
GGCATACTTGATACT20540.42981231807785586No Hit
GCATGATGAGGAACG17460.36536139599023193No Hit
ACATGCTCGTTTAGC17110.35803742757118373No Hit
CTTGTACCCGTGTGT17080.357409658849551No Hit
GTCACACCATTCAGC15080.31555841074070434No Hit
TTACTGCAAAACTTT14650.30656039239730226No Hit
GAATAGCGTGTTGAG14000.2929587367619271No Hit
AGTCTCCCTGCGGAA13470.2818681560130827No Hit
TGTCTCACAGCTCTT12760.2670109629344421No Hit
CACCTAGCGGGAAAG12430.2601055069964824No Hit
CCTAAGGTATGACGG12090.2529907948179785No Hit
ACAATCGACATTTAT11680.24441128895566488No Hit
CGAAGGTTGGGCCGT11640.24357426399348792No Hit
ATCTGCACCGTTATG11290.23625029557443974No Hit
GACTTTTTTTTTTTT11190.2341577331689974No Hit
AGCTGTTCTGTGACT9860.20632665317661436No Hit
CGGTTATGCTTCTAT9020.18874912897089874No Hit
GGAGACTCAGGTCTG7590.15882548657307333No Hit
CATGGATGATAACTT7390.15464036176218865No Hit
CATACAGAGTGTTTT7280.15233854311620207No Hit
TAGTGGCTGTTCATG7180.15024598071075973No Hit
ATGCCAATAGGAGAG6950.14543308717824238No Hit
GCAGTAGTAATTGTT6680.13978316868354806No Hit
TCAGTGGAATACAGG6560.13727209379701727No Hit
ATCTGCAACCCCGAT6340.1326684565050441No Hit
ATCTGCACTAAAACC6280.1314129190617787No Hit
TGACGGAAGGGGCGA5720.11969456959130163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)