FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515277
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG446158.65844972704002No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA353396.858252939680987No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC285735.545172790557311No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA273315.304137386299019No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA202443.9287606471858827No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG193363.752544747776439No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA167813.2566949427201295No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG154813.004403456781498No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC150802.9265812368881203No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT102391.987086557327418No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA99771.936240119392094No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT95071.8450270437065888No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA92201.7893288464262909No Hit
TCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCC83871.6276682250517684No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA83661.6235927472019902No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG72251.4021584506973919No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC71481.387215031914873No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC69801.3546112091166498No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC67091.3020181378171352No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC67061.3014359266957385No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA64761.2567997407219806No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG57771.1211445494365166No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC57051.1071714825229924No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT50510.9802494580584811No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA49760.9656941800235602No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA45980.8923355787275582No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC41780.8108260217320005No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC38290.743095461276168No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT31100.603558862514725No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG29520.5728957434544915No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA28750.5579523246719725No Hit
CAACAGTCTCCAGAATAAGGACGGAGCATTTTCCCCTGACCCTGGAGTCT28280.5488310171034221No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC26090.5063296052414527No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC23320.45257211169914435No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT22160.4300599483384665No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA21530.41783351478913283No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA18200.3532080803140835No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT17140.3326366206913951No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA16960.3291433539630141No Hit
CGATTCTCAGGGCGCCAGTTCTCTATCTCTCGCTCTGAGATGAATGTGAG15880.30818375359272776No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC15340.29770395340758465No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA15060.29226998294121415No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG15020.2914937014460184No Hit
CCCGAATGGCTACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCC14720.2856715902320499No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC13520.2623831453761763No Hit
CTGCAGAGAGGCCTAAGGGATCTTTTTCCACCTTGGAGATCCAGCGCACA13140.25500847117181635No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC12190.23657178566091636No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG11820.22939118183035534No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG11680.22667419659717006No Hit
GGGGTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG11300.2192995223928101No Hit
TCTCAGCTCAACAGTTCAGTGACTTGCACTCTGAACTAAACCTGAGCTCT11140.21619439641202692No Hit
CTCCGCACAACAGTTCCCTGACTTGTACTCTGAACTAAACCTGAGCTCTC10640.20649087772207958No Hit
TCTCAGCTCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCT9990.19387630342514803No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC8970.17408112529765543No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT8960.17388705492385648No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATCCAGCGCACA8770.1701997178216765No Hit
NNNNNNNNNNNNNNN8530.16554202885050176No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG8200.1591377065151365No Hit
CTCAGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA8150.1581673546461418No Hit
GTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAGC8090.1570029324033481No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT7900.15331559530116812No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG7570.14691127296580286No Hit
CAGACCCCAGGACCGGCAGTTCATCTTGAGTTCTAAGAAGCTCCTTCTCA7200.13973066913524182No Hit
GGGGTACAGTGTCTCTAGAGAGAAGTAGGAGCGCTTCTCCCTGATTCTGG7060.13701368390205657No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACA6790.131773783809485No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGG6590.12789237633350606No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT6470.12556353184791869No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT6090.1181888576435587No Hit
GGAACGATTTTCTGCTGAATTTCCCTAAGAGGGCCCCAGCATCCTGAGGA6030.11702443540076501No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG5930.11508373166277555No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGC5650.10964976119640504No Hit
GGACAAGTTTCTCATCAACCATGCATGCCTGACCTTGTCCACTCTGACAG5610.10887347970120924No Hit
GGTACAGCGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAGAG5440.10557428334662716No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC5280.10246915736584401No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA5240.10169287587064822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)