FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences521085
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA7741114.855733709471583No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA5603010.752564360900813No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA349986.716370649702064No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA342196.566874886055059No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA236584.540142203287371No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC213534.097795944999376No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG172443.3092489708972628No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG169313.2491819952598906No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA148582.8513582237063053No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA114372.1948434516441657No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC100831.9350010075131696No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA97761.8760854754982392No Hit
TCTCAGCTCAACAGTTCAGTGACTATCATTCTGAACTGAACATGAGCTCC90531.7373365189940222No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA76461.4673229895314583No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTCTCCTCTCACTGTGACA74421.4281739063684427No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG70831.3592791962923516No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG63421.2170759089208096No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA53041.0178761622384065No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT42600.8175249719335618No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC41970.8054348138979246No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC38850.7455597455309594No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA37990.7290557202759627No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT37590.7213794294596851No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC32670.626961052419471No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC30580.5868524329044206No Hit
GCAGAGAGGCTCAAAGGAGTAGACTTCACTCTCAAGATCCAGCCTGCAGA28320.5434813897924523No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA25900.497039830353973No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC24870.47727338150205817No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT23540.4517497145379352No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC19190.36827005191091666No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC18480.354644635712024No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG16710.3206770488499957No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA15810.3034053945133711No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA15630.29995106364604623No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA13730.26348868226872774No Hit
CTGCAGAGAGGCCTAAGGGATCTTTTTCCACCTTGGAGATCCAGCGCACA12460.23911645892704644No Hit
GCTAAGATGCCTAATGCATCATTCTTCACTCTGAAGATCCAGCCCTCAGA12380.23758120076379097No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTCTCCTCTCACTGTGA12060.23144016811076887No Hit
CTCAGCAGAGATGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG11590.22242052640164273No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT10700.20534077933542513No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA10670.20476505752420432No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG10380.19919974668240306No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTGTATC10320.19804830305996143No Hit
CCTGAATGCCCCAACAGCTCTCTCTCAAACCTTCACCTACACGCCCTGCA10240.1965130448967059No Hit
TCTCCACTCTGAAGATCCAGCCTGCAGAGCTTGGGGACTCGGCCATGTAT9620.18461479413147566No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG9480.18192809234577853No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTCTCCTCTCACTGTGA8900.17079747066217604No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT8060.15467725994799314No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC8030.15410153813677233No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT7390.14181947283072818No Hit
NNNNNNNNNNNNNNN6200.11898250765230241No Hit
CTCAGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA6150.11802297130026772No Hit
CGATTCTCAGGGCGCCAGTTCTCTATCTCTCGCTCTGAGATGAATGTGAG6140.11783106402986077No Hit
GGGGTACAGTGTCTCTAGAGAGAAGTAGGAGCGCTTCTCCCTGATTCTGG5620.10785188596869992No Hit
CTGCAGAGAGGCTCAAAGGAGTAGATTCCACTCTCAAGATCCAGCCTGCA5430.10420564783096807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)