FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466335
Sequences flagged as poor quality0
Sequence length15
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN446859.582167326063882No Hit
TCCATGCAAAAGATA342027.334212529619265No Hit
TGGTACCGGGGCGTC320476.872098384208777No Hit
TCACTGACTCTTCTT157863.3851201389559007No Hit
GCAGTAGGTTTAGGG127792.740304716566417No Hit
GGATCTAGGCAATAC127622.736659268551578No Hit
CCTGATGGAAATGTA127472.7334426967737784No Hit
CATGCTGCCTTTATG115512.4769747070239205No Hit
TATAGGCACTAAACG97442.0894850268583745No Hit
CTATGCCTGAGCAGT96612.0716866630212185No Hit
AAGCCTTAATTTTTG93782.0110006754800733No Hit
TGACGGATTCGGCGG84971.8220806930639992No Hit
TTACTGCGGCATCTT83741.7957048044860455No Hit
AGCTGGACGCTGGTC81621.7502439233598164No Hit
TCGTGAGGAATACAT81451.7465984753449773No Hit
AGCTGGATAGCAGGT75721.6237254334330469No Hit
CTAAGCGTGAACTAT70931.5210095746619918No Hit
TGACACATTTGACAT64061.3736905872387877No Hit
ACGATGCTACGCTGC63431.3601809857720308No Hit
AGCACTGGGACCTTT62451.339166050157076No Hit
CGAGATTAATTTCGT51971.1144349019481703No Hit
TCGTAGCCACGGGGC51771.1101461395777712No Hit
GGCATACCAAGTCAC46460.9962794986436788No Hit
ACGGTAGGCCCGGGT45880.9838420877695219No Hit
GAGTCTTTCCGTACG43660.9362368254580934No Hit
TGAGCAGTATCGTTT42680.9152218898431385No Hit
GATCACAATGGGCGT40920.8774807809836277No Hit
ACTCTTGGATAGGCA40400.8663299988205904No Hit
GGCGATTCTTGTATT39670.8506760161686342No Hit
CTACGTAGGCCAGGT38630.8283744518425595No Hit
CACAGATGTGAGTCC38390.8232279369980808No Hit
AGGCTAGTTTCAGCC37420.8024274395016459No Hit
GGCGATTTATACTAT36400.7805547514126111No Hit
TCAACGTTGCTGTTT33490.7181532589233062No Hit
GGAACCTCGGACGGC20830.44667460087705185No Hit
GTCGTCAAGCCATAA20200.4331649994102952No Hit
CTAAGCGGCGTCTTG16410.35189295249123487No Hit
CTGTCCATTAGCATT14860.3186550441206429No Hit
CCGTGTAGGGCTGCT14160.3036443758242465No Hit
CCGTGTAATTAATCT13700.2937802223723289No Hit
GTCGTCATCATGGAG12490.2678332100314152No Hit
CATGTGCGAGTTTTT11810.2532514179720587No Hit
CGTGTCTTCCGATCT11510.24681827441646026No Hit
TACTAGCGCGTTGAG10840.23245092047562374No Hit
GTTGCACGCCGGTCT9580.2054317175421103No Hit
GCAACTAGCCGAGGC9030.19363762102351315No Hit
TCTAGTCCGGGGGGC8910.19106436360127377No Hit
GTAACGTATACCATC7620.16340184631220045No Hit
TGGCACCGGGGCGTC7180.15396656909732273No Hit
TATCGCGGGGAAGTT6690.14345910128984526No Hit
AGCTGGAATCGTCTG6530.14002809139352612No Hit
GCAGTAGGGTTAGGG5530.11858427954153129No Hit
GAGTCTTGGCGTGAT4870.10443136371921471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)