FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443647
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA5057811.400505356736323No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA393828.876877337162203No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG352847.953169975228053No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG341117.6887705766070775No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA207674.680973837307589No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG197844.459401280747982No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA171503.865686007118272No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA135033.0436360439718966No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG134763.037550124310544No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAATTCCAGCCTGCAAA112412.5337712190097084No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA105782.384328080658722No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC98932.2299260448058926No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA72111.6253913584448898No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC71951.621784887534459No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC66211.4924027436227452No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA57091.2868339017281758No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC49991.1267967550777984No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC42440.9566164089918335No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA41090.9261868106850717No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA38880.876372431234743No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC30540.6883851350285249No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT26820.6045346863610033No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG21190.47763199120021094No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAATTCCAGCCTGCA21160.47695577790450516No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG20600.4643331297179965No Hit
TCTCCACTCTGAAGATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTAC18400.4147441546995697No Hit
NNNNNNNNNNNNNNN18350.41361713254006No Hit
GCAGAGAGGCTCAAAGGAGTAGACTTCACTCTCAAGATCCAGCCTGCAGA18280.41203930151674645No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC17600.3967118001474145No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT16970.38251132093759227No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT15860.35749142899647696No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA15800.3561390024050653No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC13540.30519760079522684No Hit
GCCTGATGGATCAAATTTCACTCTGTAGATCCGGTCCACAAAGCTGGAGG13140.2961814235191492No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGG13010.293251165904424No Hit
GGGGTACAGTGTCTCTAGAGAGAAGTAGGAGCGCTTCTCCCTGATTCTGG12160.2740917891927591No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC9410.21210557041972558No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGC9190.20714667291788288No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA8960.2019623709841383No Hit
CTCAGCAGAGATGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTG8870.19993373109702084No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT8820.19880670893751112No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA8180.18438082529578698No Hit
CGATTCTCAGGGCGCCAGTTCTCTATCTCTCGCTCTGAGATGAATGTGAG8090.18235218540866951No Hit
GGAACGATTTTCTGCTGAATTTCCCTAAGAGGGCCCCAGCATCCTGAGGA8000.18032354552155205No Hit
TCTCCACTCTGAAGATCCAGCCTGCAGAGCTTGGGGACTCGGCCATGTAT7660.17265979483688607No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT6980.15733229346755417No Hit
GCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTACGAA6880.15507824914853474No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT6630.14944313835098627No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA6600.14876692505528044No Hit
CTGCAGAGAGGCCTAAGGGATCTTTTTCCACCTTGGAGATCCAGCGCACA6560.1478653073276727No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT6420.14470964528104552No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC6360.1433572186896339No Hit
CGATTCTCAGGGCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAA5880.13253780595834075No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGAGGACTCGGCCCTGTATC5600.12622648186508642No Hit
GGACAAGTTTCTCATCAACCATGCATGCCTGACCTTGTCCACTCTGACAG5570.12555026856938062No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATCCAGCGCACA5070.1142800469742836No Hit
GATCCATCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCC4840.10909574504053898No Hit
GCTAAGATGCCTAATGCATCATTCTTCACTCTGAAGATCCAGCCCTCAGA4780.10774331844912734No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACA4630.10436225197059823No Hit
TCTCCACTCTGACGATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTAC4570.10300982537918661No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA4520.1018828032196769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)