FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478833
Sequences flagged as poor quality0
Sequence length15
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN218294.558791896130801No Hit
GGTATGCCCATAGCT164683.4391948758753053No Hit
TCCTGTATTTTACGT162253.388446493871559No Hit
TCTAGTCTTTTGGTT145423.036966959253017No Hit
ATTCGGGAAAGTGAT139772.9189717500673513No Hit
TCCTGTATGTGCGTT138032.88263340245973No Hit
AGTTCGCGTATGTGG136902.859034360622597No Hit
TAACAGCATACAAGA123542.580022680141093No Hit
CGCTTGATCCACTCG121372.534704166170669No Hit
TTGCACCGGGAGGAA108642.2688494736160627No Hit
GAAGTCCTTAGGAAG93291.9482784185718194No Hit
TCCTGTAGTCACCGC87921.8361307595758856No Hit
GATAGTCAGGATTAG86111.7983305244208314No Hit
GCTTAAGTATTCCGG77501.618518356086569No Hit
ACATCCGAGATACCC76291.593248585623798No Hit
GATGCTCAGTTGTGG69881.4593814544945733No Hit
CGTACTCCACACCTT68821.4372443002048731No Hit
AGCCTTCGCGGACCG67591.4115568475856928No Hit
GATGCTCTCCCGATG63721.3307353503204666No Hit
GTCCAAGCGACGTGC62681.309015878187176No Hit
GGACTAATCAGGGTT60951.2728863716577596No Hit
GATGCTCGTACGTCC60441.262235476669319No Hit
TCCTGTAATCGTCCC59431.2411425277706425No Hit
TGAAGCTAGTTTTAG59181.2359215008155244No Hit
CGCTTGAGGACCACT56711.1843377544989588No Hit
AGTGTACCAATATGG54901.146537519343905No Hit
CAGCGATAATGTTCC53661.1206412256465197No Hit
GTTCGGATGTAGATG52341.0930742033234968No Hit
GCTACTCGAGCGGCT51741.0805437386312138No Hit
CAGCGATTATGTTGG50601.056735855715876No Hit
TTAGGCACTGTTGCT50191.0481733715094823No Hit
CGCATTGCTCTTAGA49651.0368959532864275No Hit
GTCCAAGCGGGTGCA48741.0178914151697982No Hit
TGAAGCTTTAGTTCA48221.007031679103153No Hit
GTTCGGACACTCTAA48031.0030636986172632No Hit
GGCACTTGTGTACAC42340.8842331251187784No Hit
ACTGCAGGTACAAGG33540.7004529762986261No Hit
ACCAGTGTTGGAAAA33420.6979468833601694No Hit
AGCAGGTGCTGGGCC31760.6632792643781862No Hit
TCAGTGGGACGGGGC31660.6611908535961389No Hit
TAGACGCGCGGGAGG31500.6578493963448635No Hit
GACACCTCTAGCGAA27860.581831243878346No Hit
TCTGGAGTGGCGTGC24230.5060219324900331No Hit
AATGCCGATTGGTGG22750.4751134529157347No Hit
TATAGGCTTTATCGG22310.4659244454747271No Hit
GACCTGTTCACCAAT21650.45214093431321567No Hit
TCTACAGGTGTAATC20320.42436507091198805No Hit
TAATGCCCGTCGACT20260.42311202444275975No Hit
TACCGGAACAATCGC20200.42185897797353145No Hit
GATCGCTTTGCCGGA19210.40118371123126434No Hit
ATGACTTAGCAGTTT15700.32788049278140813No Hit
GGTGATCACTGAAAT12160.25395075109693777No Hit
CGAACTTGCCAATTT11670.24371753826490655No Hit
TGCCAGAGTTAAATT11550.24121144532644992No Hit
GTGGTACGTGTTACA11490.23995839885722162No Hit
ATGCGCACGCATGGC10350.21615051594188367No Hit
TACTGACGGCGCGAG10340.21594167486367896No Hit
GATCACAGTCCAGTG9920.2071703495790808No Hit
CGGTTATTGGAGGAA9790.20445541556241947No Hit
ATGACGTTTTTCTGG9650.2015316404675534No Hit
AGATCGAGGCAACTC9590.2002785939983251No Hit
CGCTACAGGCGGAGT8850.1848243542111759No Hit
GTTCATGTCATACAT8420.17584418784837302No Hit
CCTGATGATAGATGG8000.16707286256377485No Hit
CTAGAGTATTAACTC8000.16707286256377485No Hit
CTAGAGTGATAGTTG7860.1641490874689088No Hit
GACGTAGGCTAGGCA7350.15349819248046814No Hit
AGATCGAACTGTTGC7320.152871669245854No Hit
GCCGTAATATAACTT7310.15266282816764926No Hit
TACTGACCGGTACTT6840.14284729749202751No Hit
GTCCAAGCGGGGGCA5670.11841289134207542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)