FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401965
Sequences flagged as poor quality0
Sequence length15
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN388229.658054805766671No Hit
AGCCTTCTGTATTTT129503.22167352878982No Hit
CTACGATGTTGGGGG101792.5323100269923997No Hit
CCATGGTATCCCGGG86522.1524262062617394No Hit
GTTGACTGAAGAGGG85322.1225728608212155No Hit
ATGAGCGTCGAAAGG78461.95191123605289No Hit
CTACGATCATGAAGG73181.8205565161145871No Hit
CTACGATCTGGTCCT73041.8170736258131925No Hit
CCATGGTGGCCGGGA71351.7750301643177888No Hit
CCATGGTGGGTGAGT69991.741196372818529No Hit
ATCGTGAGAGCCGGG67951.690445685569639No Hit
TTCCTGAACCGCTTG65441.628002438023211No Hit
CATGCTGGATGCGTG59111.4705260408244498No Hit
CCATGGTACTTGATG59011.4680382620377397No Hit
CTTGTACCGACAAAC57931.4411702511412685No Hit
ATGAGCGCTCATTTT57231.4237557996342964No Hit
GTCGTCAGCAGGCAA55311.375990446929459No Hit
GGAACCTTTTGCTTG53851.3396688766434888No Hit
CGACGTTCTGGTAAT45971.1436319082507185No Hit
GACACCTGCTTGACC43581.0841739952483425No Hit
GACACCTTATTGCTT43191.0744716579801723No Hit
GTTAGGCGACGCGGT43061.071237545557449No Hit
TGACGGAACAGCATT41581.0344184195141368No Hit
TCCGCATATTCATAT39560.984165288022589No Hit
GAAGTCCATTAAGAC39100.9727215056037217No Hit
CGATGATGAACCGGA38330.9535656089460526No Hit
CTTGTACCATTAACC38230.9510778301593423No Hit
ACTGTCCTCCCGTGG38080.9473461619792768No Hit
TTCAAGCATCTTCGC37160.9244585971415422No Hit
GTTGCTACGGCCCAT34190.8505715671762467No Hit
CAGTCAAGAATGGAA32560.8100207729528691No Hit
GTAACTGGTGACCTA29960.7453385244984017No Hit
TGACATGCGTTCTGT28090.6988170611869192No Hit
CTTACGGGCCGTATG26760.6657296033236724No Hit
CTGATCAGTAGTTTT26260.653290709390121No Hit
CTACGTAGACAAGTA25700.6393591481845434No Hit
GATGTACCATTAAGA25530.6351299242471359No Hit
CATCTGGTAATGTGT25230.6276665878870051No Hit
CAGGAATTATGGCAG25100.6244324754642817No Hit
CGCAATCGCTTTTGC22700.5647257845832349No Hit
GCTGACACGTATGCG22460.5587551154951301No Hit
CCGAAGTAAGATCTA21590.5371114400507507No Hit
TTCAGGAATAATTCG21530.5356187727787245No Hit
CGAGTAGAAGGAATT20650.513726319455674No Hit
GTAACTGAACTTGTC20200.5025313149154778No Hit
GCCATAGCCGCACCG20120.5005410918861095No Hit
CTATGCCGTTTAACC19260.479146194320401No Hit
TCAGTGGTAGCATTT18720.4657121888721655No Hit
CAGCACTTCCTGTTC18660.4642195216001393No Hit
CATGCTGGCTTTCTT18520.4607366312987449No Hit
CATCTGGAATGGCCC18420.4582488525120347No Hit
ACCGATCGGTTGTTT18250.45401962857462713No Hit
AACCTTGGGTAGGGT17680.4398392894903785No Hit
CTCGATCGCGGGAAG17550.4366051770676551No Hit
TTCATGGTTTGAGTT17140.42640528404214295No Hit
CGTCCAAGCGTAAAA15190.3778935977012924No Hit
CGTCCAACTGGGGTC14880.3701814834624905No Hit
GACGTAGATGATGGT14560.3622205913450176No Hit
ACTCTTGAGTCGTAG14550.3619718134663466No Hit
CATGAGGGTGTGTGC12860.31992835197094277No Hit
AACCTTGGTTTCATG12470.3102260147027726No Hit
TGCTACTTTGTAAAG12440.30947968106675955No Hit
CGATGATGTTCAAGG11950.29728956501187914No Hit
CGCAATCAGTGATAG11790.29330911895314266No Hit
ACTGTCCTGTGTAGT11770.2928115631958006No Hit
GTAAGTCTTCGTTTG11670.29032378440909035No Hit
CTTAGTGGGGGTCTT11560.28758722774370904No Hit
CTAGAGTAATGCCGG10780.26818255320736883No Hit
CTACGATGTTGGGGT10710.2664411080566716No Hit
CTTGTACTTTAGTGG10510.26146555048325104No Hit
GAGATCGGTATGAAG10400.2587289938178697No Hit
CTGTCATACAGGTTT10390.2584802159391987No Hit
ACCGATCCTCGGGGT9590.23857798564551638No Hit
CTGTGGACTTTTAGT9530.23708531837349023No Hit
TCTCATGGATTCTGC9460.23534387322279304No Hit
CCGAAGTACCCCCGG9420.23434876170810892No Hit
CATTGGACGGCCGGT8960.2229049792892416No Hit
CTCTGGATTTTCTTT8880.22091475625987336No Hit
CAGGAATTGGTTGAG8720.21693431020113693No Hit
GTAATCCTTAATTAT8550.21270508626372941No Hit
TCTCATGACGGTTGG8380.20847586232632195No Hit
GCATGATTTGTCGTT8300.2064856392969537No Hit
CTTCGAAGAGGGCGC7870.1957881905140995No Hit
TTGCCAGGGTATTTT7450.1853395196099163No Hit
CTTGACCATCAGGTG7370.18334929658054808No Hit
TAAGGCTTGCTGATT7300.18160785142985086No Hit
CATTGGAAATCATAT7270.18086151779383777No Hit
CGATGATTGGGAGGT6490.16145684325749754No Hit
ATGGCTCGGGATGAT5770.1435448359931835No Hit
TGAATCCTCGCGGAA5340.13284738721032924No Hit
CGCAATCTAGAGAAG5000.12438893933551429No Hit
CTTACGGCCAGTCCG4660.11593049146069931No Hit
CATGCTGGCCATTTT4500.11195004540196286No Hit
TGAGACCCCGCCGCT4480.1114524896446208No Hit
AGGTTCGATTTGTTT4380.10896471085791051No Hit
AACCTTGGGTGGAGA4190.10423793116316098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)