FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369251
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA4362111.813373558907085No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA4065211.009313448033993No Hit
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA3712410.053865798603118No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA342309.270117074835275No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC221355.994567380995583No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA134473.6416962987236325No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG118223.201616244776588No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA86342.3382468835561716No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAGA77782.1064262520616057No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA76972.0844899539879376No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC59941.6232860574514354No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT53851.45835759415682No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG51481.3941736109042357No Hit
CAGATGGCTATAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT50931.3792785936937204No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA50641.3714248573463579No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG50511.3679042169147817No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA42281.1450205957465247No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA39071.0580878589360625No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC36180.9798213139571728No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA32750.8869305702625044No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG31580.8552448063783172No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGG24000.6499643873679422No Hit
GGAACGATTTTCTGCTGAATTTCCCAAAGAGGGCCCCAGCATCCTGAGGA23460.6353401886521635No Hit
CAGATGGCTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACT20870.565198198515373No Hit
TGGCTACAATGTCTCCAGATTAAAAAAACAGAATTTCCTGCTGGGGTTGG20570.5570736436732737No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA18350.4969519378417391No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC16490.4465796978207236No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGAGCACA16490.4465796978207236No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC16260.44035087244178084No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA13090.3545014096102651No Hit
GGTACAGCGTCTCTCGGGAGAAAAGGAATCCTTTCCTCTCACTGTGACAT10830.2932964297997839No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC9970.2700060392524326No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT9800.26540212484190967No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC8370.22667508009456983No Hit
GGGGTACAGTGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCACTCTGG8330.22559180611562324No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC8330.22559180611562324No Hit
TCTCAGCTCAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCT7350.1990515936314323No Hit
TCTCAGCTCAACAGTTCAGTGACTTGCACTCTGAACTAAACCTGAGCTCT7260.1966142271788025No Hit
TACAAAGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACATC7190.194718497715646No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA6950.18821885384196657No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC5990.1622202783472489No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT5810.15734554544198934No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC4390.11888931918938607No Hit
GATCCATCTCCACTCTGACTGTGAGCAACATGAGCCCTGAAGACAGCAGC4310.11672277123149294No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC4130.11184803832623338No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC3830.1037234834841341No Hit
CCCTGATGGCTACAATGTCTCTAGATCAAAGACAGAGGATTTCCTCCTCA3800.10291102799992416No Hit
CAGATGGCTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACT3730.10101529853676769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)