FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432380
Sequences flagged as poor quality0
Sequence length15
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN283176.54910032841482No Hit
TAGAGCCCAGGGGAT117322.7133539941717935No Hit
AACGTTCCCGCTTTG107762.4922521855775015No Hit
CGTCCAATATCTGGC73151.6917988806142745No Hit
CTAGTGCATATTGCT69271.6020630001387668No Hit
CGTCAGATATAGATG68971.5951246588648875No Hit
CAACATGGGTATGCT67921.5708404644063094No Hit
GCTGAATGCGTCCGA66591.540080484758777No Hit
CTTCAGTAGTAGTTT66561.5393866506313891No Hit
TGCAAAGTGGCGAGT64781.4982191590730376No Hit
TAGAGCCGGTCAGTC64741.497294046903187No Hit
TAGAGCCATTCGGTG59961.386743142606041No Hit
GCTGAATTACCTTTT59231.3698598455062676No Hit
AGATCACGTGGTCGA59121.3673157870391786No Hit
CGGTTATCTGAGTTA57641.3330866367547065No Hit
CGTCCAATGCGCTTG55281.2785050187335214No Hit
TGCACGTGCTAGACA54541.2613904435912855No Hit
CGTCCAAAAGACAGT54151.2523705999352421No Hit
GATGTACGTTCGTTA48921.131412183727277No Hit
CTAGTGCACGGGGAT46781.08191868264027No Hit
TGGACACGGGTGGTT44651.032656459595726No Hit
AGGCTAGATTTCGTT44191.0220176696424441No Hit
CTTCAGTTATTTTCT43951.0164669966233406No Hit
GATGCTCCTTGAGGG41870.9683611637911097No Hit
CAATGCGGCAGTCTG39870.92210555529858No Hit
ATTCGCGCAGTCCGT39770.9197927748739535No Hit
GTTGCACGAGACTGC39110.9045284240714188No Hit
GGACATTCGTGCAGG39010.9022156436467923No Hit
CTAGTGCATGCTCTT38530.891114297608585No Hit
GGTCTAGTATGTGTT36130.8356075674175494No Hit
TCTCATGTGGGAATA35730.8263564457190434No Hit
TGACGTCGGGGCCGG34340.7942087978167354No Hit
GATGCTCGGGGCGGT34060.7877330126277812No Hit
CGGATTACAGCCATG32790.7583607012350247No Hit
GCAATTGGGTCGGCT31500.7285258337573431No Hit
TCTTCGATTGTGCCG29490.6820389472223507No Hit
AGCCTTCGTTTGTGT28620.6619177575281003No Hit
TACGTACTTGAGTTT28070.6491974651926546No Hit
ATAACCGGGGCAAGG27940.6461908506406402No Hit
CTTGACCAGATATTG27170.6283824413710162No Hit
AGGTTCGACTATTGT26510.6131180905684814No Hit
CAATGCGGTTTCACC25800.5966973495536334No Hit
ACATCCGTGTACGCT25050.5793514963689348No Hit
TGGACACCGGTTTCG24960.5772699939867709No Hit
AGGAGTCAAGGGTCG24520.5670937601184143No Hit
GGACGATCAGGTGAT24110.5576113603774457No Hit
CGTCCAAAAGGGCGT24100.5573800823349832No Hit
CTTGACCTCCGCCGG23940.5536796336555807No Hit
GTCCAAGGCATCGGT23690.5478976825940145No Hit
ACGATGCCTAGCAAT23560.5448910680420002No Hit
ACGTTGGGGGTCTAA23240.5374901706831954No Hit
TCACTGAGCGATGTC23120.5347148341736435No Hit
CTGATCAGCTGTATT22970.5312456635367039No Hit
GTAACGTTGCATTTT22580.5222258198806605No Hit
AAGGTACCCGTTTGG22580.5222258198806605No Hit
GCTTAAGTTTGAAAC22120.5115870299273787No Hit
GCCGTAATATAAATC22030.5095055275452148No Hit
GATGTACTGTAATGA21940.5074240251630511No Hit
ACTCAGGAGGGCCAT21540.498172903464545No Hit
ACGTTGGACTGACCT21520.4977103473796198No Hit
CTGATCAGTGCCGTA21370.4942411767426801No Hit
TCTTCGACATGTTCT21250.4914658402331283No Hit
CTTCATAATCTCTCT20560.47550765530320555No Hit
CTGATCAGCACGAAG20500.4741199870484296No Hit
GTCATGCTCCAAGTG19840.4588556362458948No Hit
GCTTAAGTTAGGTTT19470.4502983486747768No Hit
AGGCTAGGGCCGTCT19350.447523012165225No Hit
GGAATCGTGTGATTC19280.4459040658679865No Hit
CTTACGGGCGTAGGC19190.44382256348582266No Hit
AAGACTCTACGGGAC18100.4186132568573939No Hit
GCTTAAGCAGACTAC18020.41676303251769276No Hit
GACCTGTGGTAGCAT17620.4075119108191868No Hit
CTTACGGTCTAGGGC17590.40681807669179887No Hit
CTGATCAGCCGAGTT16450.38045237985105695No Hit
ACTTGGCATGGTCTT16010.37027614598270037No Hit
GTCGTCAACGGGACA15920.36819464360053655No Hit
GTCGTCAGCTCGCGT15910.3679633655580739No Hit
CGAGTAGGCTTGGAT15580.36033119015680654No Hit
TCACAAGGGCGATGT15180.3510800684583006No Hit
CATGAACCAACCCGT14970.34622322956658497No Hit
CTGATCATTATCTTG14180.3279522642120357No Hit
GGAATCGTTCCTCTT14030.32448309357509597No Hit
ACGTCAAGGGGTGAT13590.31430685970673944No Hit
GTTAGCGGGGTGATT13060.30204912345621904No Hit
CTTGACCGTGTCAAT12650.2925667237152505No Hit
CTTCGAACATAGATT12220.28262176788935656No Hit
TCAGTGGGTGTTTGT12130.28054026550719274No Hit
TTCATGGTGACCCTT11960.2766085387853277No Hit
GAGGATCGCCGGAGC10940.25301817845413754No Hit
GGACATTGCAAGGAT9860.22804014986817153No Hit
GAATAGCGGGGAAAT9450.21855775012720294No Hit
TGGACACCGGACCCC8980.20768768213145847No Hit
TCAACGTGGACGGCT8910.20606873583421992No Hit
AAGGCTAAGTTGGGT8640.1998242286877284No Hit
AGTCGACCCCACTTT8330.1926546093713863No Hit
GTCGAGATGCGGCCA7980.18455987788519357No Hit
CACAGATGGGTGCCT7700.1780840926962394No Hit
AGTCGACGTGCCGTT7550.1746149220592997No Hit
TGTCACGGGTCGAAT7340.1697580831675841No Hit
TCAACGTTTTGGGGC7170.16582635644571905No Hit
GCCATTCTTACTTGT6790.1570377908321384No Hit
GCATCCAAGCATATG6760.15634395670475046No Hit
ATCTGCAGGTGATTT6670.15426245432258662No Hit
ACGCTTCAAACCGTT6580.15218095194042278No Hit
TAGACGCTCACGGTA6550.1514871178130348No Hit
TCCTGTAGTGCTAAT6030.1394606596049771No Hit
GAGCTAGAGCTGGCT5930.13714787918035062No Hit
CATGAACTTGAAGTG5930.13714787918035062No Hit
GGCGATTTAGATAAT5760.1332161524584856No Hit
CGCAATCTGTCATCT5720.132291040288635No Hit
ATCTACGGCCAATGT5530.12789675748184468No Hit
GATAGTCCGAAAGTC5450.12604653314214348No Hit
GAGGATCGCGATGAT5390.1246588648873676No Hit
CTACAGCGATTGTTT5260.12165225033535317No Hit
GATGCTCGGGGCGGG5250.1214209722928905No Hit
CTAGTGCATGTTTCT5120.1184143577408761No Hit
AGCACTGCGCCCTGC5050.11679541144363755No Hit
GTCAGTGCTGTATCC4850.11216985059438456No Hit
CTTAAGCATCGTGTC4500.10407511910819187No Hit
TGATCCGTTTAGTTC4490.10384384106572923No Hit
CCGCATTGGGTGGCT4350.10060594847125214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)