FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002271254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372454
Sequences flagged as poor quality0
Sequence length15-160
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA19061351.17759508556762No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG4752612.760233478496675No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA192305.163053692536528No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT181794.880871194832113No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA135773.6452823704403765No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT60411.6219452603542988No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC57091.5328067358653685No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC53351.4323916510495256No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA38931.0452297464921843No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA32940.8844045170678796No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA29830.8009042727424057No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT21420.5751045766725555No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA19580.5257025028594136No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC15710.42179705413285934No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA14700.3946796114419499No Hit
CCTGAATGCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAG7320.19653433712619545No Hit
NNNNNNNNNNNNNNN6890.18498928726768943No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG6420.17237027928281076No Hit
CGATTCTCAGGGCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAA5630.15115960628695085No Hit
GCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA5600.15035413769217137No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC5270.141493983149597No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC5110.13719815064410637No Hit
GCTGAAAGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA4460.11974633109055079No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA4050.10873826029523109No Hit
CCTGAATGCCCCAACAGCTCTCTCTTCAACCTTCACCTACACGCCCTGCA3810.10229451153699518No Hit
CCTGAATGCCCCACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCAG3730.10014659528424986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)