FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372454
Sequences flagged as poor quality0
Sequence length15
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGACATCTGCCT286557.693567527802091No Hit
ATCGTGAGTAGAGTT274047.357687123779044No Hit
GGAACCTAGTCTAGT268827.217535588287413No Hit
GCATGACCACGCAAT265357.124369720824585No Hit
GTTACCTTGGAGCTA251106.741772138304328No Hit
CGTACTCCGTCCTGT214375.755610088762639No Hit
ATCAGGCCGATTCGG142253.819263586912746No Hit
AACAGCTCTTCGTGT142083.8146992648756624No Hit
CGCAATCAGAGATAC139683.750261777293303No Hit
TTCAAGCAGCAGAAG130883.5139909894913197No Hit
NNNNNNNNNNNNNNN128583.4522383972248925No Hit
GTTACCTGTTCTAGC80922.1726172896518765No Hit
GAAGTCCTAGAAGAT79122.124289173965107No Hit
AGATACCTTAGACTA68421.8370053751604225No Hit
GGATCTACGAGTCAC53071.424873944164917No Hit
GAGCTGAGTTGAGAT46331.2439119998711252No Hit
TCGTGAGCGTAGATT36070.9684417404565395No Hit
CATGAACGTCCGCGC34460.9252149258700403No Hit
CAAGGTTCTCTTCCT32540.8736649358041529No Hit
GCATCAGGGCTTTTT31960.8580925429717495No Hit
CGTATGATGAGCGTT31920.8570185848453769No Hit
TGCGTATGGTTCGTT31490.8454735349868708No Hit
TGCGTATTGAGGCTA31150.8363448909127034No Hit
GCCTACAGTACTGGT31130.835807911849517No Hit
ATCAGGCATCATGAT27550.7396886595391646No Hit
AGCTAGTCACCCTTT24790.6655855488194515No Hit
ATTCCGTCGGAATTG21680.5820853044939778No Hit
CGCATATATCCGGCT17390.4669032954405108No Hit
CACGAGTTCCCTAGG11920.32003952165905053No Hit
GTTACCTGGTCTGGG11380.30554108695301974No Hit
TAGCCGAATATTATT9520.25560203407669135No Hit
CATTGGACTGAGCAA5750.15438148066606883No Hit
ATCGTGAGTAGCGTT5450.14632679471827395No Hit
CGCATATTCTTAGGG5250.14095700408641068No Hit
GGCACCTAGTCTAGT4740.12726403797515934No Hit
GCATGACCACGCATT4740.12726403797515934No Hit
TCGTGAGGCACGGGA4550.12216273687488924No Hit
GTTACCTTGGAGCTT4520.12135726828010976No Hit
ATCGTGCGTAGAGTT4310.11571898811665332No Hit
TCATGACCACGCAAT4110.11034919748479007No Hit
AGTCGACCTCTGCCT3940.1057848754477063No Hit
CTCGTGAGTAGAGTT3830.1028314906001815No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)