FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416620
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATGCCCCAACAGCTCTCTCTTAAACCTTCACCTACACGCCCTGCA9668823.207719264557632No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA6063814.554750132014785No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA4969011.926935816811483No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG322467.739906869569392No Hit
GCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTACGAA161813.883874993999328No Hit
GATAACTTCCAATCCAGGAGGCCGAACACTTCTTTCTGCTTTCTTGACAT123262.958571359992319No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA112112.6909413854351687No Hit
GTTTCCCATCAGCCGCCCAAACCTAACATTCTCAACTCTGACTGTGAGCA109672.6323748259805098No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA100672.4163506312707024No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT89952.1590418126830206No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG80741.937977053429984No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCGGCC80491.931976381354712No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA64951.5589746051557776No Hit
CAGACCCCAGGACCGGCAGTTCATCCTGAGTTCTAAGAAGCTCCTTCTCA50431.2104555710239546No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA49621.1910133935000722No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCGGCCGTGTAT32380.7772070471892852No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG25540.6130286592098315No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA20660.49589554030051364No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCA18230.43756900772886564No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA18170.43612884643080024No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA16650.3996447602131439No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG6630.15913782343622487No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC6580.1579376890211704No Hit
GGGGTACAGTGTCTCTAGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA6300.15121693629686525No Hit
CTCAGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA5220.12529403293168836No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGGGACA4760.11425279631318709No Hit
NNNNNNNNNNNNNNN4680.11233258124909991No Hit
GCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTCCGAA4240.10177139839662043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)