FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445930
Sequences flagged as poor quality0
Sequence length15
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN310396.960509497006257No Hit
AGTCTCCGTCACTGG289496.491826071356491No Hit
AGTCGACGTTCATCT244655.486287085416993No Hit
CATGAACCCGCGATT237925.335366537348911No Hit
AGGAGTCGTGTCAGC188834.234521113179198No Hit
TAGCCGAGTGTTTAT167373.753279662727334No Hit
GAGTCTTCAGACCTG157983.542708496849281No Hit
CTATCGTCATTGATT144793.2469221626712716No Hit
GGTGATCCGCGAGTC139853.136142443881327No Hit
GCTACCAGACTCCTT135353.0352297445787455No Hit
ATCAGGCAGTAATCT131842.956517839122732No Hit
ACAGGTACATGTCTG110942.4878344134729664No Hit
TGTCACGCGAGAGAT98412.206848608526002No Hit
GGTATGCCTCGACGT95572.1431614827439285No Hit
CTAGTGCGTGTTGGC95052.131500459713408No Hit
ATCAGGCAGAGTGCT94152.1113179198528917No Hit
GATCGCTCGGGATTT90802.0361940214831926No Hit
TCTTCGACAAGCGCG81461.826744107819613No Hit
TCACTTGAGTACATT78911.7695602448814838No Hit
GAGACTAGCCTGTGG77681.7419774404054449No Hit
CGAGTAGGGAAAACA74941.6805328190523174No Hit
AATCACGAACCGCAG62161.3939407530329873No Hit
TACGACTGAGTGCCC53421.1979458659430853No Hit
ACATGCTCGGGGGAG43110.9667436593187272No Hit
CATGAGGGAGTTTTA37110.8321933935819523No Hit
GTTCAACGTCTTGTA32700.7332989482654229No Hit
ACATGCTTGCGGCTG30860.6920368667728118No Hit
CGAACTTCTCGTTTG30630.6868791065862355No Hit
ATGACGTTATGGCGA27640.6198282241607427No Hit
GCCATTCACCAACTT26760.6000941851860158No Hit
CGACACTGCGTTTTT24130.5411163187047294No Hit
TCGACTCAAGGACTG23920.5364070594039423No Hit
CAGGAATTCGTGGGG22980.5153275177718476No Hit
ATCGCCATCCAGTAT21840.48976296728186036No Hit
TGACGGAGACGGACT21680.4861749601955464No Hit
AAGGTACCATTCGTT13420.30094409436458636No Hit
GTAACGTCTAGTCTT12700.2847980624761734No Hit
GTCCAAGCAAATAAT12680.28434956159038416No Hit
CTACCGACGTGGTAG12200.27358554033144217No Hit
ATGACGTAACGTGTG10600.2377054694683022No Hit
ATGGCTCGACGCGAA10360.2323234588388312No Hit
CTTGCAGAGGTGGAG9120.20451640391989773No Hit
GGTCTTAACGGCCGT9040.20272240037674077No Hit
CATGAACCCGCGCTT5930.13298051263651245No Hit
ATAGCGAGGGACATA5410.12131948960599198No Hit
CAATCGAAATGGGGT4460.10001569753100262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)