FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408311
Sequences flagged as poor quality0
Sequence length15
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN4890511.977389783767766No Hit
TGACACACTGGGTTT228445.594754978435555No Hit
GAACCTCTGGCAGCC211515.180120055545895No Hit
CGAATTCTAGACATT191174.681970360827898No Hit
CGGTATTAGATGTCT161193.9477261205306737No Hit
CTGTGGAGTGGCAGG158773.888457572781532No Hit
AAGCCTTCCGCGCCG153473.7586545549838237No Hit
TCAGCCTGAACGCTC153433.7576749095664823No Hit
CTCGATCGTCGTGGC118442.9007300807472736No Hit
CGGTATTGGTACTGG109912.6918206954992643No Hit
TGACACATAGGTAGC106942.6190820232616803No Hit
AGGACATAGTCTAGC100152.452787213668013No Hit
TAGGCATGCAGAGAA97602.3903348183125117No Hit
GTTCAACATGTGTTC89472.1912218872379143No Hit
TGAGACCAATTAGTT84102.0597044899598593No Hit
AGTGAGCCCTTTATA78351.9188804612170625No Hit
AAGTGCATTGTAAAA63951.5662081109742327No Hit
AACGTTCTTCGTATT58511.4329763342158306No Hit
GTTCAACCTTTTGAG56611.386443176892124No Hit
CTGATCACTGTGGGG48931.1983512567626147No Hit
TCTTCGACCGGGGAT43341.061445809689183No Hit
CCATGACCTGGACAA41981.0281378654995823No Hit
CACAGATATTGGTAG38770.9495213207579517No Hit
CGTATGATAAGTCAT37410.9162133765683511No Hit
TAATGCCGGACGAGA34200.8375968318267204No Hit
GCTACCAGGGCCGGG32150.7873900041879841No Hit
GCTTGTACGTCTCTT21500.5265594118208914No Hit
CATGCTGTGTTAGGT15040.3683466769202887No Hit
ATTCGCGTTGGTTCT13080.3203440514705702No Hit
GCAGTAGTGGGTTTT10440.25568745392605147No Hit
CTGTGGCGTGGCAGG9620.235604722870557No Hit
GCAGTAGGTGCTTGG8270.2025416900352917No Hit
TGAGACCTGTGATGA7700.18858174283817972No Hit
CAGCCTTCCGCGCCG7280.1782954659560972No Hit
CGGCATTAGATGTCT6870.16825410042834996No Hit
CGGCATTGGTACTGG6080.1489061034358614No Hit
CGACTTCTAGACATT5750.14082402874279654No Hit
TGACACCCTGGGTTT5750.14082402874279654No Hit
CGGACATAGTCTAGC5590.13690544707343177No Hit
TGACACAATATCGAC4700.11510833653759023No Hit
CGGTCTTATTCAGAT4620.11314904570290785No Hit
CTGTGGAGTGGCCGG4610.11290413434857255No Hit
CGAACTTGAATAAGG4300.10531188236417828No Hit
CGGTCTTAGATGTCT4220.1033525915294959No Hit
CGAATTCTAGACCTT4210.1031076801751606No Hit
CTGCGGAGTGGCAGG4190.10261785746649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)