FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424064
Sequences flagged as poor quality0
Sequence length15-160
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG240125.662352852399638No Hit
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA196494.633498717174766No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC180704.261149260488983No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG179674.236860473890734No Hit
GATCCATCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCC137173.2346532598853006No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG120612.8441461666163597No Hit
GGTACAGCGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGACA119932.828110851192273No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA99242.340212798068216No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA93832.2126377150618772No Hit
GCTAAGTGCCTCCCAAATTCACCCTGTAGCCTTGAGATCCAGGCTACGAA88722.0921370359191065No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC83641.9723437971626925No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA65351.5410409749471778No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC49381.164446875943254No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC43821.0333345910051313No Hit
TGGATACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG43111.0165918351946877No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG42491.0019714005433142No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC42070.9920672351343194No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT31970.7538956383942047No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC30470.7185236190763659No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC30000.7074403863567763No Hit
GGGGTACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA27780.6550897977663749No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA22480.5301086628433445No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAGCCTGCAAA20970.4945008300633867No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG20430.48176690310896464No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC19070.44969627226079084No Hit
TGGATACAGTGTCTCTCGGGAGAAGAAGGAATCCTTTCCTCTCACTGTGA18590.4383772260790824No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATTCAGCGCACA18010.4247000452761847No Hit
CAGACCCCAGGACCCATTAAGCCACCCCCGTGTTCCTGCCGTCAGTGCCA17470.4119661183217628No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC17300.407957289465741No Hit
CGATTCTCAGGGCTGCCCAACGATGACTAATCAAACTAACCTCAAAACAA17090.4030052067612436No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG16460.3881489586477513No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG16170.38131036824630243No Hit
GGACAAGTTTCTCATCAACAACCGACTAATCACCACCCAACAATGACTAA16130.3803671143978267No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATTCAGCGCACA15840.37352852399637787No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC15100.35607832779957743No Hit
TCTCCACTCTGACGATTCAGCGCACAGAGCAGCGGGACTCAGCCATGTAT14490.341693706610323No Hit
CGATTCTCAGGGCGCCATCGCCAAGTAAGAAAGTGAAGCAAATCAGAAAC12990.30632168729248416No Hit
GTTTCCCATCAGCCGCCCAAACCAGGGCTATGAAGCAGGTAGCAGGTACC12780.30136960458798673No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC12540.2957100814971325No Hit
TCTCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACT11960.28203290069423487No Hit
GATCCATCTCCACTCTGACAGTGACCAGTGCCCATCCTGAAGACAGCAGC11940.281561273769997No Hit
GGGGTACAGTGTCTCTCGACAGGCACAGGCTAAATTCTCCCTGTCCCTAG11690.27566593721702387No Hit
GTTTCCCATCAGCCGCCCAACAATGACTAATCAAACTAACCTCAAAACAA11040.26033806217929367No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG10420.2457176275279203No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG10270.24218042559613642No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG9830.2318046332629037No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA9530.22473022939933596No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG9490.22378697555086022No Hit
NNNNNNNNNNNNNNN9450.22284372170238453No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA8360.19714005433142168No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT8300.19572517355870814No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG8260.19478191971023243No Hit
GTTTCCCACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC8140.19195215816480532No Hit
CTGCAGAGAGGCCTGCCTTCCGGATTCCAGGCGGGCATGCCCTGCAAACC7790.18369868699064293No Hit
CCTGAATGCCCCAACAGCCCTCCTGTGCCTGCTTCCCTGTGTGTCGGGGT7160.1688424388771506No Hit
CAGACCCCAGGACCGCCCTTAGAAGGCTGCGCCGGCCCGCGAGAGGTCTG6950.16389035617265318No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA6930.16341872924841533No Hit
GATCCATCTCCACTCTGACATCCGGCCTGCTTCTTCTCACATGACAAAAA6890.16247547539993962No Hit
TCTCCACTCTGAAGATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTAC6800.16035315424086932No Hit
CAACAGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCG6730.15870246000603683No Hit
GATCCATCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCC6530.1539861907636583No Hit
ATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC6460.15233549652882583No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC6440.15186386960458798No Hit
CGATTCTCAGGGCGCCCAACGATAGCGACTCTGTCTCAGGGGATCTGCAT6350.14974154844551765No Hit
CAGACCCCAGGACCAGCTCAGGACAGATGCCACACAAGGATAGATGCTGG6280.14809085421068519No Hit
CGATTCTCAGGGCGCCCAACGATCTATAAACCTAGCCATGGCCATCCCCT6270.14785504074856626No Hit
CGATTCTCAGGGCGCCCAACGATCCTAGGCCTACCCGCCGCAGTACTGAT5990.14125226380923636No Hit
GTCTCTAGATTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCAT5630.13276297917295504No Hit
CACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTA5610.1322913522487172No Hit
CAGACCCCAGTCCACCATGATCCATCTGGGTCACATCCTCTTCCTGCTTT5170.12191555991548446No Hit
CGATTCTCAGGGCGCCCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCA5140.12120811952912768No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT5130.12097230606700875No Hit
TCTCACCTAAATCTCCAGACAAAGCTAAGACCCCCGAAACCAGACGAGCT5020.11837835798370058No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT4830.113897902203441No Hit
CAGATGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGAT4810.11342627527920314No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA4800.11319046181708423No Hit
CCTGAATGCCCCAACAGCAGCGCGGGGCCGCCAAGGAGGCCGCGGGGAAG4800.11319046181708423No Hit
GCTGAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATG4740.11177558104437064No Hit
CAGACCCCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAAT4640.10941744642318141No Hit
CCCTGATGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGG4610.10871000603682464No Hit
ATTTCTCATATGATGTTAAAAATATTAAACACAAACTACCACCTACCTCC4560.10753093872623001No Hit
CAGACCCCAGGACCTGTGCAATCAAATATTGTGGAGAATTCCCTAGCTGG4490.10588024449139752No Hit
GGGGTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC4440.10470117718080289No Hit
GATAACTTCCAATCCAGGAGGCAGTGGAGGAAGTGGGGGTTATGGTGGGA4420.10422955025656505No Hit
CAGACCCCTTGTGAAGCCCAAGATCGTCAAAAAGAGAACCAAGAAGTTCA4340.10234304255961363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)