FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424064
Sequences flagged as poor quality0
Sequence length15
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN289136.8180746302444915No Hit
GGATCTATGGATGGT143543.3848664352550557No Hit
GTTCAACCTTTATTC142123.351380923634168No Hit
ATGCTACTATTAGTG117762.776939329912466No Hit
GGACATTGGCAGGGA114912.7097324932085725No Hit
CAATCGACAGCCGGC93802.2119302746755207No Hit
CTGTCCAGTTATAAC90232.127744868699064No Hit
CGACGTTGTCGATAT63081.4875113190461817No Hit
TGCACGTTGTTTTTT61271.4448290824026562No Hit
CGCAATCAAATGAGG46921.106436764261998No Hit
TAACAGCTCAGGGTT40730.9604682312103834No Hit
TATAGGCGGCCACCG36550.8618982040446724No Hit
ACAGGTAACAGTGCA33410.7878527769393299No Hit
TGCCTAAGGGTGGAG31560.7442272864473287No Hit
GACTGTGTGTTGTTC30820.7267770902505282No Hit
CGCAATCTTTGGGTC21890.5161956685783278No Hit
CTACCGATAACCAAT18870.4449800030184123No Hit
GGACATTGGCAGGGG16020.37777316631451857No Hit
ATGACGTTCGTTAAA12770.3011337911258678No Hit
AGTCTCCATTGATTT12470.2940593872623No Hit
ATGAGCGGCGAGGCG9520.22449441593721703No Hit
GCCGTAATGGTTTAC9070.21388281014186536No Hit
AGTACCTGTTATTTT9030.21293955629338968No Hit
CGAAGGTGTTGTGTA8880.20940235436160579No Hit
GGATCTATGGATGGG8320.196196800482946No Hit
CGAAGGTAGTTGCAT7430.1752094023543616No Hit
TAACAGCAGGGCATA7240.17072894657410204No Hit
GGAACCTTTAGAGGA7130.16813499849079383No Hit
CCTGACTCAATTATC6720.1584666465439179No Hit
GGCTCTATGGATGGT6710.15823083308179897No Hit
GGATCTATGGCTGGT6570.15492944461213404No Hit
TTGCTACTATTAGTG6560.15469363115001508No Hit
GGACATTGGCAGGGT6170.145496906127377No Hit
ACGTTGGTGGGACGT6050.1426671445819499No Hit
GTCGAGAGCGATTGT5950.14030900996076062No Hit
GTAGTCTACGGGTAT5890.13889412918804708No Hit
GGATCTCTGGATGGT5810.1370076214910957No Hit
GGATCTATGGTTGGT5440.12828252339269544No Hit
TATAGGCCGGTCATG5080.11979323875641412No Hit
GGATCTATGGGTGGT4960.11696347721098703No Hit
ACAGGTAGGTTTTGG4680.1103607002716571No Hit
TCTTAGGGCTCCATG4570.10776675218834894No Hit
TTGTCCAGTTATAAC4530.10682349833987322No Hit
GGTCAACCTTTATTC4410.10399373679444612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)