FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288769
Sequences flagged as poor quality0
Sequence length15
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTCGGGCGGGT175136.064709161994535No Hit
NNNNNNNNNNNNNNN146605.076722224338485No Hit
TCGCCAATGTATGTC110143.814121321887045No Hit
CCGTGTACCCCATCG105623.657594824929269No Hit
CTGTCCAACTAGTTT101253.506262791366109No Hit
CGGATGTGGGAATCG98543.412416152703372No Hit
GAGATCGGCATTATG92153.191132012092711No Hit
CGGATGTGGGGGGTT85552.9625756227295867No Hit
TGACATGAGGCAGCA73202.5348981365728314No Hit
GCAACTAGTTTAGAT72012.4936887269755412No Hit
CAGCACTCTACCAAG61652.1349244551873645No Hit
CTGATGGTGTGTGCA61252.1210725528017202No Hit
CGGATGTAGAGCGTT60072.080209440764071No Hit
TCTAGTCACAGTTTT58822.0369222458089338No Hit
ACTTGGCTAGAACTC55211.9119088267784978No Hit
CCTGACTAAATGTTG52621.8222177588314534No Hit
GAGATCGTTACCTAC43251.4977369454477454No Hit
CAGGAATTTGAAGGT38661.3387863655724819No Hit
GCATGGAACGTCACA36101.250134190304361No Hit
ATAACCGGTTATTAG34801.2051155075510183No Hit
TAGACGCAAGGATGG32971.1417430541366975No Hit
CATACAGTACGAGGC32031.1091910835304344No Hit
CTAGCTCGGGCGGGG15910.5509594173889857No Hit
CGGATGTGGGGGTTT15250.5281037784526732No Hit
ATAGCTCGGGCGGGT13970.4837776908186128No Hit
CTGTCCATGGTTCCT12390.4290626763953195No Hit
CGTCCAAGGCTCGTA9900.34283458404468625No Hit
ATGTCCAACTAGTTT8540.295738115933497No Hit
CGAGCTCGGGCGGGT7800.27011209652005586No Hit
ACGTGTACCCCATCG7750.2683806087218503No Hit
TATAGGCACGTCGGT7300.25279721853800097No Hit
TCGCCACTGTATGTC5460.1890784675640391No Hit
CTGATGGGGTGTGCA5120.17730435053624177No Hit
AGGATGTGGGAATCG5060.1752265651783952No Hit
AGTCGACCGAGTTAT4790.16587653106808556No Hit
GCAACTCGTTTAGAT4320.149600545764954No Hit
CGGATGTGGGCATCG4310.14925424820531288No Hit
CAGCGATGGGACGTC4270.1478690579667485No Hit
AGGATGTGGGGGGTT4240.14683016528782522No Hit
CTAGCGCGGGCGGGT4210.14579127260890193No Hit
AAGCACTCTACCAAG4030.13955791653536218No Hit
ATGATGGTGTGTGCA3780.13090047754433476No Hit
GCAACTAGGTTAGAT3740.12951528730577036No Hit
ACTGACTAAATGTTG3740.12951528730577036No Hit
CTAGATCGGGCGGGT3560.12328193123223061No Hit
TCGCCCATGTATGTC3470.12016525319546074No Hit
CGGATGCGGGAATCG3240.1122004093237155No Hit
CGGCTGTGGGAATCG3210.11116151664479221No Hit
CTGTCCCACTAGTTT3190.11046892152551001No Hit
CTAAGCGGAAGCTAG3170.10977632640622782No Hit
CTGACCAACTAGTTT3170.10977632640622782No Hit
CTGGCCAACTAGTTT3130.10839113616766342No Hit
CGGATGCGGGGGGTT3120.10804483860802233No Hit
CGGATGTGGGGGGGT3090.10700594592909904No Hit
CTAGCTCGGGAGGGT3030.10492816057125247No Hit
CGGCTGTGGGGGGTT2900.10042629229591818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)