FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371687
Sequences flagged as poor quality0
Sequence length15
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGCCCGTTAA11464230.843693753077183No Hit
ACGCTTCGGTCGCGT306448.244571373225321No Hit
NNNNNNNNNNNNNNN198665.344819700446882No Hit
TGTCACGTAACATGA106392.862354615577085No Hit
TGGCTATCTTGATTT101402.728101870659991No Hit
CCATTGCAGGGCTGT88992.3942187916176785No Hit
GATCACAGTGTATTT70301.8913763462267985No Hit
CGACTTGGAGTAATT68881.8531721582944791No Hit
CTTGTACGCAATGTG57591.5494219598748409No Hit
GAATCTGGCTTGTCT54541.4673636688934506No Hit
CGGTTATCCAAGTGT49351.3277300524365931No Hit
TGAAGCTTTCTGGGA49061.3199277887039365No Hit
AGGCATGGCACGGGG44251.190517828172629No Hit
ACATCCGAGGGCCCT31620.850715790436577No Hit
AAGCCTTAGCAGGTT23660.6365571031539978No Hit
ATTCCAGCCCGTTAT17960.4832022642707439No Hit
CGAGTAGTAGTGCAA17380.4675977368054304No Hit
ACGATTCGGTCGCGT11900.32016185661591606No Hit
ACGCTTCGGTCGCGG11100.2986383704568629No Hit
ACGCTGCGGTCGCGT10760.2894908888392653No Hit
AGGACATGTATGTCA10750.2892218452622772No Hit
ACGCTTCGGGCGCGT10640.2862623659154073No Hit
CCATGACTGTTGCAC10010.2693126205651529No Hit
CGAGTCTGGCAGGTA9920.2668912283722595No Hit
GCGCTTCGGTCGCGT9600.2582818339086382No Hit
ACTACTGTCGAGACG9470.25478426740779203No Hit
GGGCTATCTTGATTT9270.24940339586802876No Hit
ACGCGTCGGTCGCGT8670.23326078124873886No Hit
CCGCTTCGGTCGCGT8020.21577294874450814No Hit
GTTCAACATGGGACT8020.21577294874450814No Hit
ACCGATCGAAGGGTA6780.1824115451979757No Hit
ACGCCTCGGTCGCGT6400.17218788927242545No Hit
ACGCTTCGGTAGCGT6120.16465466911675683No Hit
ATGTCGCTTGGGTAT5940.15981188473096986No Hit
ACGCTTAGGTCGCGT5660.15227866457530126No Hit
TGTCACGTAACATGG4820.12967900410829541No Hit
GTTCCAGCCCGTTAA4810.12940996053130727No Hit
GGTCACGTAACATGA4800.1291409169543191No Hit
ATTCCAGCCCGTTAG4520.12160769679865047No Hit
GTCCATAACAACGGT4370.11757204314382799No Hit
ACGCTTCGGTGGCGT4110.11057691014213572No Hit
GACACCTTCATGGCT4090.1100388229881594No Hit
GTGCTATATTTTAGG4010.10788647437225407No Hit
TGTCACGGAACATGA3970.10681030006430142No Hit
ACGCTTCGGTCGAGT3820.10277464640947895No Hit
GCATTGCAGGGCTGT3740.10062229779357362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)