FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271276

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271276
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373578
Sequences flagged as poor quality0
Sequence length15-160
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGAATGCCCCAACAGCTCTCACTTATTCCTTCACCTACACACCCTGCA259206.938310071792237No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA253476.784928448677386No Hit
CTCTGAGCTGAATGTGAACGCCTTGGAGCTGGACGACTCGGCCCTGTATC184434.93685388325865No Hit
CGATTCTCAGGGCGCCAGTTCTCTAACTCTCGCTCTGAGATGAATGTGAG179844.813987975737329No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG137363.6768760473047126No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC136353.6498401940157077No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC135953.6391329253863987No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG119193.190498369818351No Hit
CTCCGCACAACAGTTCCCTGACTTGCACTCTGAACTAAACCTGAGCTCTC105902.8347493696095594No Hit
CTCTGAGCTGAATGTGAACGCCTTGTTGCTGGGGGACTCGGCCCTCTATC90042.4102061684574574No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC72531.9414954842094556No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAATCTATACGAGATACTCCAGCCAA37300.9984527996830649No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC36890.9874778493380232No Hit
CGATTCTCAGGGCGCCCAACGATGACTAATCAAACTAACCTCAAAACAAA34540.9245726461408328No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA31530.8440004497052823No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC30480.8158938695533463No Hit
CTGCAGAGAGGCCTGAGAGATCCGTCTCCACTCTGAAGATCCAGCGCACA28640.7666404338585249No Hit
CGATTCTCAGGGCGCCAGTTCTCCGGCGGGAAGGAAAACCGCGCAGAGAG25050.6705426979104766No Hit
CCTGAATGCCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAGGAGGAG24400.6531433863878494No Hit
GTTTCCCATCAGCCGCCCAAACCTTAGAAAGCTGGATGATACAGACCCTG23970.6416330726113423No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG22860.6119204021650098No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGGGGGACTCAGCTGTGTAT22440.6006777701042352No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC22300.5969302260839771No Hit
CCTGAATGCCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGA17340.46416009508054545No Hit
CCTGAATGCCCCAACAGCTCAGTGCTTTCCTGACACTCCAGTGTCTGGGG17080.4572003704714946No Hit
GATAACTTCCAATCCAGGAGGCCGGCACGGAGGTGGTCAAGGCTAAAGCC16260.43525046978141113No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG15610.417851158258784No Hit
CCTGAATGCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC15470.4141036142385258No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC15120.40473475418788046No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA13780.3688654042796953No Hit
GTCTCTAGATTAAACACAGAGGATTTCCCGCTCAGGCTGGAGTTGGCTGC13360.3576227722189208No Hit
CGATTCTCAGGGCGCCAGTGAAGAAATCTATACGAGATACTCCAGCCAAA13110.35093072932560265No Hit
CGGTTCTTTGCAGTCAGGCTTCATACGCTATTGTCCTGCCCGTTAGAGCA13050.3493246390312063No Hit
GTTTCCCATCAGCCGCCCAAACCAGGGCTATGAAGCAGGTAGCAGGTACC12970.34718318530534453No Hit
CCTGAATGCCCCAACAGCTCTCACGGCTGCTGCTCCTCTGGACTCCCCAA12360.33085460064564826No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGC12200.3265716931939247No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGCCACACTCGCA12130.3246979211837956No Hit
CAGACCCCAGGACCAGCTGACAGCACTCACTGGGCGGATCCAGGAGGCCG12040.3222887857422011No Hit
CGATTCTCAGGGCGCTCTCCCAGCAAAGCAGTGGCCGCCCGGGCGGCGGG11690.3129199256915557No Hit
GGAACGATTTTCTGCTGAATTTCCCAACCCACCCCCAAACTAAACACTAA10180.27249998661591424No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA9430.25242385793595984No Hit
CGATTCTCAGGGCGCCCAACGATTAACTGAATATTCACGAACACTGAGTG9250.24760558705277078No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG9150.24492876989544352No Hit
CGATTCTCAGGGCGCCCAACGATCTATAAACCTAGCCATGGCCATCCCCT7430.1988875147894148No Hit
TCTCCACTCTGAAGATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTAC7340.19647837934782028No Hit
GTTTCCCATCAGCCGCCCAAACCTCACTAGACATCAGATAATCCATACTG7220.19326619875902756No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG7120.19058938160170033No Hit
CAGACCCCAGGACCGGCCCGTGGTACCCAGCAGGTGGCACTGCAGCTCCC6530.17479616037346954No Hit
GTTGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGGAGTTGGC6300.16863948091161685No Hit
GTGTCTCCAGAGCAAACACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCT6190.16569498203855687No Hit
CAGACCCCAGGACCGTGCAGCATATAAAGAGTACATCTCCAATAAACGTA6140.16435657345989324No Hit
TCTCACCTAAATCTCCAGACAACCTTAGCCAAACCATTTACCCAAATAAA5890.15766453056657512No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGGGGGACTCAGCTGTGTAT5820.15579075855644606No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC5760.15418466826204968No Hit
CGATTCTCAGGGCGCCCAGTGAACTGCAATGAAAAGATCGTGGTCCTTCT5490.1469572619372661No Hit
TCTCACCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAG5430.14535117164286976No Hit
GCAGAGAGGCTCAAAGGAGTCAACATTTAGATCTGGAAGGGACAAGTCAT5430.14535117164286976No Hit
CAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC5420.14508348992713702No Hit
GTTTCCCACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC5380.14401276306420613No Hit
CAGATGGCTATAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG5360.1434773996327407No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGG5350.14320971791700796No Hit
CGATTCTCAGGGCGCCCAACGATCCTAGGCCTACCCGCCGCAGTACTGAT5280.1413359459068789No Hit
TCTCAGCTCAACAGTTCAGTGACTTGCACTCTGAACTAAACCTGAGCTCT5120.13705303845515526No Hit
CAGATGGCTACAGTGTCTCTAGATCAACCACAGAGGATTTCCCGCTCAGG5080.13598231159222438No Hit
CGATTCTCAGGGCGCCACCCTTGACGCTTGCAGCTTCGGAGTCACGGGTT4950.13250244928769897No Hit
CGGTTCTTTGCAGTCAGGCCTGTTATAACCGCTGAGCAGTCACACTCGCA4850.12982563213037168No Hit
CAGACCCCAGGACCGGCAGCCATGGCGGTGGAAGGAGGAATGAAATGTGT4700.12581040639438082No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC4620.12366895266851903No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA4610.12340127095278629No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG4350.11644154634373545No Hit
CGATTCTCAGGGCGCCCAGTGAAGAAAGGACAAGAATCCTTCAAGAAACA4120.11028486688188276No Hit
ATTTCCCCCTCTATTGATCCCCACCTCCAAATATCTCATCAACAACCGAC4060.1086787765874864No Hit
CAGACCCCAGACATCAAGCTCTTTGGGAAGTGGAGCACCGATGATGTGCA4040.10814341315602095No Hit
GATCCATCTCCACTCTGAAGATCCAGCGCACAGAGCAGGGGGACTCAGCT3970.1062696411458919No Hit
GGTACAGCGTCTCTCGGGACAACTGGCACAAGCGCCGCAAAACCGGGGGC3920.10493123256722828No Hit
CAGACCCCAGGACCGGCTTCTGGGCGGCTGAAAGGGGCAAGGAGGCAAGG3800.10171905197843556No Hit
CGATTCTCAGGGCGCCAGTTCCCTAACTATAGCTCTGAGCTGAATGTGAA3790.10145137026270283No Hit
CGATTCTCAGGGCGCCAGCCCAGGCCTGAGGAAGCAGCGGAATTCCAGGC3750.10038064339977194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)