FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002271277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373578
Sequences flagged as poor quality0
Sequence length15
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNN274507.347863096863305No Hit
ATGACGTTGTATCTT160024.283442815155068No Hit
CGGTCTACAGTCCAC151964.067691352274492No Hit
GCAATTGTCAAAGGT121453.2509944375739472No Hit
GAGATCGTCGGGTGT95612.559304884120585No Hit
TCGAAGGAATATAGT94712.53521352970464No Hit
TGAAGCTGTAAGGGC69041.8480745654187347No Hit
GCATGGATCGAGAGT61641.6499900957765177No Hit
ATTCCGTAGGGTGAT56011.4992852898189937No Hit
ACTGTCCGAACTACG55671.490184111484081No Hit
GGTCCATTTCGAGCG49811.333322626064704No Hit
GGACTAATCCGTTAT43051.1523697862293818No Hit
GAGATCGGTGCTAAA35020.9374213684960035No Hit
CTAGACGGTTACGTA33300.8913801133899748No Hit
CTGACGTTGTATCTT15770.4221340657105076No Hit
CGAGTAGCGCCGGAG14430.3862647158023224No Hit
GAGATCGTCGGGGGT12180.3260363297624593No Hit
GCATGGATAGTCAGC9750.26098967283940705No Hit
AATCACGGAGTTTTA9180.2457318150426417No Hit
TCGACTCAACAAAGT8050.21548378116484374No Hit
ATGCCGTTGTATCTT7900.2114685554288529No Hit
GCAATTGTCAAAGGG7810.20905941998725835No Hit
TCGCAGGAATATAGT6860.18362965699264946No Hit
CCTGTCCGAACTACG6770.18122052155105495No Hit
GCACTTGTCAAAGGT6090.16301816488122964No Hit
GCATCCATTATGCCG6060.16221511973403144No Hit
CGGCCTACAGTCCAC6010.16087671115536784No Hit
GATAGTCCTGATCAC5900.15793221228230786No Hit
CGGTCTACAGGCCAC5860.15686148541937694No Hit
GAGCTCGTCGGGTGT5750.15391698654631697No Hit
AGTCGACACGGATGC5530.14802798880019702No Hit
CGATATCTTCCGTCT5470.14642189850580067No Hit
ATGACGATGTATCTT5010.13410853958209532No Hit
CTTCCGTAGGGTGAT4940.13223476757196623No Hit
GGCACTTTTTCAGGC4920.13169940414050077No Hit
CTTCGAATGTATATT4910.13143172242476805No Hit
TGACGCTGTAAGGGC4910.13143172242476805No Hit
GAGATCGTCGGGTGG4890.1308963589933026No Hit
ATGACGGTGTATCTT4450.1191183635010627No Hit
AACCTTGCAGGGCTG4360.11670922805946816No Hit
TCGACGGAATATAGT4330.11590618291227No Hit
CGGACTACAGTCCAC4240.11349704747067547No Hit
GAGATCGTCGGGCGT4120.11028486688188276No Hit
CGGGCTACAGTCCAC4110.11001718516615006No Hit
ACTGGCCGAACTACG4090.10948182173468458No Hit
ATGACGTTGGATCTT4050.1084110948717537No Hit
ATGACGTTGTCTCTT4030.10787573144028825No Hit
ATTCCGTAGGGGGAT4000.10707268629309007No Hit
GAAGTCCATTATTGG3930.10519891428296098No Hit
TACGACTCGTGGATT3910.10466355085149553No Hit
GCGATCGTCGGGTGT3870.10359282398856462No Hit
ATGACGTTGTGTCTT3770.10091600683123739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)