FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002271278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002271278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367236
Sequences flagged as poor quality0
Sequence length15-160
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC8166922.238832794170506No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCACA4636612.625668507444804No Hit
GGGGTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG298258.121480464878172No Hit
GCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCAAA177444.831770305743445No Hit
GCTGAAAGGACTGGAGGGACGTATTCTACTCTGAAGGTGCAGCCTGCAGA121123.298151597337952No Hit
GCCTGATGGATCAAATTTCACTCTGAAGATCCGGTCCACAAAGCTGGAGG108482.9539587622128547No Hit
CAGATGGCTATAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACT98782.6898234377893235No Hit
GATCCATCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCC90982.4774259604178237No Hit
CAGATGGCTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACT87972.395462318509079No Hit
GCTAAGATGCCTAATGCATCATTCTCCACTCTGAAGATCCAGCCCTCAGA74552.0300297356468318No Hit
CGATTCTCAGGGCGCCCAACGATGCTCTTAGCTGAGTGTCCCGCGGGGCC39291.0698842161443867No Hit
TCTCCACTCTGACGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT29580.8054765872626867No Hit
GGGGTACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG28660.7804245771111764No Hit
TCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT27990.762180178413881No Hit
TACAAAGTCTCTCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTC24270.6608829199751658No Hit
GTCTCTAGATTAAACACAGAGGACCTCCCCCTCACTCTGGAGTCTGCTGC23350.6358309098236556No Hit
CTGCAGAGAGGCTCAAAGGAGTAGACTCCACTCTCAAGATCCAACCTGCA21290.5797361914409263No Hit
TCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC20900.5691163175723513No Hit
TGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCAGGCTGG20840.5674824908233398No Hit
TGGATACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG19550.5323552157195918No Hit
GTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGCTGTCGGCTGC19530.5318106068032546No Hit
CGGTTCTTTGCAGTCAGGCCTGACGAGTGCTGACAGCTATGAAGAAGACC18460.5026740297792156No Hit
CTCAGCAGAGATGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA17230.4691805814244791No Hit
GGTACAGCGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGGAG16610.4522977050180265No Hit
CTGCAGAGAGGCCTAAGGGATCCATCTCCACTCTGACGATCCAGCGCACA14940.4068228605038722No Hit
CCCTGATGGCTACAATGTCTCTAGATCAAACACAGAGGACCTCCCCCTCA14440.3932076375954427No Hit
CCCTGATGGCTACAATGTCTCCAGATTAAACAAACGGGAGTTCTCGCTCA11510.3134224313520461No Hit
CGGTTCTTTGCAGTCAGGCCTGAGGGATCCGTCTCTACTCTGAAGATCCA11100.30225794856713395No Hit
TCTCCACTCTGAAGATCCAGCGCACAGAGCAGCGGGACTCGGCCATGTAT11010.29980720844361664No Hit
GGACAAGTTTCTCATCAACCATGCAAGCCTGACCTTGTCCACTCTGACAG10910.2970841638619307No Hit
CGGTTCTTTGCAGTCAGGCCTGAGAAACGGCTACCACATCCAAGGAAGGC9320.2537877550131251No Hit
CGGTTCTTTGCAGTCAGGCCTGACTGGCATTGTATTAGCAAACTCATCAC8380.22819113594527768No Hit
CTGCAGAGAGGCCTGAGGGATCCATCTCCACTCTGACGATCCAGCGCACA8320.22655730919626618No Hit
TGGATACAGTGTCTCTAGATCAAACACAGAGGACCTCCCCCTCACTCTGG8180.22274504678190593No Hit
TCTCCACTCTGACGATCCAGCGCACACAGCAGGAGGACTCCGCCGTGTAT7520.20477295254277902No Hit
CAGATGGCTACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT7360.2004160812120816No Hit
GATCCATCTCCACTCTGAAGATCCAGCCCTCAGAACCCAGGGACTCAGCT6730.1832609003474605No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACCTCAATTCC6310.17182411310437976No Hit
TCTCCACTCTGACGATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTAC6030.16419958827565925No Hit
TCTCCACTCTGAAGATCCGGTCCACAAAGCTGGAGGACTCAGCCATGTAC5860.15957041248679324No Hit
GTTGTCTCCAGATCCAAGACAGAGAATTTCCCCCTCACTCTGGAGTCAGC5350.1456828851201952No Hit
CGATTCTCAGGGCGCCGGCGGCTTTGGTGACTCTAGATAACCTCGGGCCG5230.14241523162217212No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCCCATCAATTCC5210.14187062270583495No Hit
CTCAGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGAGATCCAGCGCA5140.1399644914986548No Hit
TGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCAGGCTGC5090.13860296920781187No Hit
CAGACCCCAGGACCGGCCCATGTCCCCCGGAGACTGTCCTCCGGAAACAA5040.1372414469169689No Hit
CTGCAGAGAGGCCTAAGGGATCTTTCTCCACCTTGGCGATCCAGCGCACA5030.13696914245880035No Hit
CAGATGGCTATAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATT5010.13642453354246314No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCACTTCC4790.1304338354627542No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCCATTCC4760.12961692208824843No Hit
GGGGGACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG4740.12907231317191126No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTACATCTTCACATCAATTCC4660.12689387750656256No Hit
GTTTCCCATCAGCCGCCCAAACCGTGGCAGCAGGACCGACAAGTCAAGTG4230.11518478580531322No Hit
GATCCATCTCCACTCTGAAGATCCAGCGCACACAGCAGGAGGACTCCGCC4090.11137252339095297No Hit
TGGCTACAATGTCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCC4040.11001100110011No Hit
TCTCACCTAAATCTCCAGACAAAGCTCACTTAAATCTTCACATCAATTCA3910.10647104314391836No Hit
CCCTGATGGCTACAATGTCTCCAGATCAACCACAGAGGATTTCCCGCTCA3830.10429260747856964No Hit
GGGGAACAGTGTCTCTAGAGAGAAGAAGGAGCGCTTCTCCCTGATTCTGG3830.10429260747856964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)